ORK04146

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK04146

Order Kazusa clone(s) from :

Accession No	AB209156
Product ID	ORK04146
Clone name	aj00560
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	AQP4
cDNA sequence	DNA sequence (4824 bp) Predicted protein sequence (219 aa)
HaloTag ORF Clone	FHC04146
Flexi ORF Clone	FXC04146
Description	Aquaporin-4 (AQP-4) (WCH4) (Mercurial-insensitive water channel) (MIWC).

Features of the cloned cDNA sequence

Description

Length: 4824 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	4164 bp
Genome contig ID	gi51511735r_22586008
PolyA signal sequence (AATAAA,-27)	+--------+--------+----*----+---- CTTGTGAAAATAAACATTTGGATGTTTTAAAAGGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAACATTATGTTTGATGAAGTTAAAAATTCATTTGTAGTTAGATCTTAAG

Features of the protein sequence

Description

Length: 219 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92393	2.9e-92	100.0	aquaporin 4 iso...	Homo sapiens
AAC52112	8.3e-54	64.9	mercurial-insen...	Homo sapiens
XP_855456	1.6e-52	62.1	similar to Aqua...	Canis lupus fam...
XP_512074	1.2e-51	65.3	aquaporin 4 iso...	Pan troglodytes
P55087	1.2e-51	65.6	Aquaporin-4; Sh...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000425	41	137	PD000295	Major intrinsic protein
FPrintScan	IPR000425	43	62	PR00783	Major intrinsic protein
	IPR000425	84	108	PR00783	Major intrinsic protein
	IPR000425	121	140	PR00783	Major intrinsic protein
HMMPfam	IPR000425	35	196	PF00230	Major intrinsic protein
ScanRegExp	IPR000425	102	110	PS00221	Major intrinsic protein

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	45	VTAEFLAMLIFVLLSLGSTINWG	67	PRIMARY	23
	2	73	LPVDMVLISLCFGLSIATMVQCF	95	PRIMARY	23
	3	117	ISIAKSVFYIAAQCLGPIIGAVL	139	PRIMARY	23