ORK04263

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK04263

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Accession No	AB209451
Product ID	ORK04263
Clone name	ah02705
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	BEST1
cDNA sequence	DNA sequence (5762 bp) Predicted protein sequence (376 aa)
Description	Bestrophin-1 (Vitelliform macular dystrophy protein 2) (TU15B).

Features of the cloned cDNA sequence

Description

Length: 5762 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	4256 bp
Genome contig ID	gi51511727f_61374408
PolyA signal sequence (GATAAA,-29)	+--------+--------+----*----+---- TTAATAGATAAAAATCCCAGACTACTTCAGCCTTT
Flanking genome sequence (113936 - 113985)	----+--------+--------+--------+--------+----* AATGCCTTTTATTCATAAAAACTGTGAAAGCTAGACTGAACCATTGGAAA

Features of the protein sequence

Description

Length: 376 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92688	2.8e-156	100.0	bestrophin vari...	Homo sapiens
EAW73982	3.6e-110	97.8	vitelliform mac...	Homo sapiens
AAC64344	2.7e-109	97.5	bestrophin [Hom...	Homo sapiens
AAH15220	8.5e-81	96.7	BEST1 protein [...	Homo sapiens
XP_001116563	8.5e-81	96.7	similar to best...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000615	102	206	PD002802	Bestrophin
BlastProDom	IPR000615	288	310	PD002802	Bestrophin
HMMPfam	IPR000615	76	374	PF01062	Bestrophin

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	252	PLVYTQVVTVAVYSFFLTCLVGR	274	PRIMARY	23
SOSUI2	2	291	VVPVFTFLQFFFYVGWLKVGLSR	313	SECONDARY	23