ORK05364

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05364

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Accession No	AB209214
Product ID	ORK05364
Clone name	fj10387
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRM7
cDNA sequence	DNA sequence (4004 bp) Predicted protein sequence (656 aa)
Description	Metabotropic glutamate receptor 7 precursor (mGluR7).

Features of the cloned cDNA sequence

Description

Length: 4004 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2033 bp
Genome contig ID	gi89161205f_7215370
PolyA signal sequence (None)	+--------+--------+----*----+---- CTCCAGCCTGGGCAACAAGAGCAAAACTCCATCTC
Flanking genome sequence (483655 - 483704)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAAAAGAAAAAGAAAAAAAAGAGAAGAATTTATAT

Features of the protein sequence

Description

Length: 656 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92451	100.0	Metabotropic gl...	Homo sapiens
AAM47559	99.8	metabotropic gl...	Homo sapiens
Q14831	100.0	Metabotropic gl...	Homo sapiens
EAW63934	99.8	glutamate recep...	Homo sapiens
AAM47557	99.8	metabotropic gl...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000162	3	14	PR00593	Metabotropic glutamate receptor
	IPR001883	12	23	PR01057	Metabotropic glutamate receptor 7
	IPR000162	22	34	PR00593	Metabotropic glutamate receptor
	IPR000162	87	102	PR00593	Metabotropic glutamate receptor
	IPR001883	133	144	PR01057	Metabotropic glutamate receptor 7
	IPR000162	158	169	PR00593	Metabotropic glutamate receptor
	IPR001883	186	200	PR01057	Metabotropic glutamate receptor 7
	IPR001883	235	247	PR01057	Metabotropic glutamate receptor 7
	IPR000162	414	428	PR00593	Metabotropic glutamate receptor
	IPR000162	579	593	PR00593	Metabotropic glutamate receptor
	IPR001883	628	643	PR01057	Metabotropic glutamate receptor 7
HMMPfam	IPR001828	1	240	PF01094	Extracellular ligand-binding receptor
	IPR011500	274	309	PF07562	Nine cysteines of 3 GPCR
	IPR000337	349	610	PF00003	GPCR
ProfileScan	IPR000337	345	619	PS50259	GPCR
ScanRegExp	IPR000337	296	318	PS00980	GPCR
ScanRegExp	IPR000337	539	549	PS00981	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	343	SPWAVIPVFLAMLGIIATIFVMA	365	PRIMARY	23
	2	383	LSYVLLTGIFLCYIITFLMIAKP	405	PRIMARY	23
	3	415	VFLGLGMCISYAALLTKTNRIYR	437	SECONDARY	23
	4	458	LAITSSLISVQLLGVFIWFGVDP	480	PRIMARY	23
	5	510	QIICSLGYSILLMVTCTVYAIK	531	PRIMARY	22
	6	545	IGFTMYTTCIVWLAFIPIFFGTA	567	SECONDARY	23
	7	578	TTLTISMNLSASVALGMLYMPKV	600	SECONDARY	23