ORK05810

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05810

Order Kazusa clone(s) from :

Accession No	AB209402
Product ID	ORK05810
Clone name	fh23250
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	SPNS1
cDNA sequence	DNA sequence (5295 bp) Predicted protein sequence (337 aa)
Description	Linker for activation of T-cells family member 1 (36 kDa phospho- tyrosine adapter protein) (pp36) (p36-38).

Features of the cloned cDNA sequence

Description

Length: 5295 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	4217 bp
Genome contig ID	gi51511732f_28793626
PolyA signal sequence (AATAAA,-15)	+--------+--------+----*----+---- TCTTACCTTTTGTGGTTCTCAATAAAACAGAACTT
Flanking genome sequence (115973 - 116022)	----+--------+--------+--------+--------+----* AAAAAATTGTCTAAGTAACTGAAAAAGTTAATATGCAGCACAGCACTTTT

Features of the protein sequence

Description

Length: 337 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92639	8.5e-122	100.0	spinster varian...	Homo sapiens
XP_001147209	1.2e-91	100.0	spinster isofor...	Pan troglodytes
AAH47741	1.2e-91	100.0	SPNS1 protein [...	Homo sapiens
AAH06156	1.3e-91	100.0	SPNS1 protein [...	Homo sapiens
Q9H2V7	1.4e-91	100.0	Protein spinste...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR011701	132	335	PF07690	Major facilitator superfamily MFS_1
ProfileScan	IPR007114	128	337	PS50850	Major facilitator superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	120	LSPGRSALIVAVLCYINLLNYMD	142	PRIMARY	23
	2	164	SGLIQTVFISSYMVLAPVFGYLG	186	PRIMARY	23
	3	193	YLMCGGIAFWSLVTLGSSFIPG	214	SECONDARY	22
	4	216	HFWLLLLTRGLVGVGEASYSTIA	238	SECONDARY	23
	5	253	RMLSIFYFAIPVGSGLGYIAGSK	275	SECONDARY	23
	6	286	ALRVTPGLGVVAVLLLFLVVREP	308	PRIMARY	23