ORK06184

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06184

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Accession No	AB209237
Product ID	ORK06184
Clone name	fj15288
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	NPY1R
cDNA sequence	DNA sequence (4008 bp) Predicted protein sequence (262 aa)
Description	Neuropeptide Y receptor type 1 (NPY1-R).

Features of the cloned cDNA sequence

Description

Length: 4008 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1823 bp
Genome contig ID	gi89161207r_164364569
PolyA signal sequence (ATTAAA,-27)	+--------+--------+----*----+---- GACATTTTATTAAAATCAATATTGTTTTTGCTTTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TCTGAGGAGTCTCTTTCAGTTTCATTTTTTCTCATCCCATGACTTCCCTC

Features of the protein sequence

Description

Length: 262 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92474	2.2e-110	100.0	neuropeptide Y ...	Homo sapiens
AAG40771	1.8e-97	99.5	neuropeptide Y ...	Macaca mulatta
XP_001148035	1.8e-97	99.5	similar to neur...	Pan troglodytes
P25929	1.8e-97	99.5	Neuropeptide Y ...	Homo sapiens
AAX29893	1.8e-97	99.5	neuropeptide Y ...	synthetic construct

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000351	40	55	PR01013	Neuropeptide Y1 receptor
	IPR000276	50	74	PR00237	Rhodopsin-like GPCR superfamily
	IPR000611	75	87	PR01012	Neuropeptide Y receptor
	IPR000276	83	104	PR00237	Rhodopsin-like GPCR superfamily
	IPR000611	105	117	PR01012	Neuropeptide Y receptor
	IPR000611	128	143	PR01012	Neuropeptide Y receptor
	IPR000276	128	150	PR00237	Rhodopsin-like GPCR superfamily
	IPR000276	162	183	PR00237	Rhodopsin-like GPCR superfamily
	IPR000351	184	199	PR01013	Neuropeptide Y1 receptor
	IPR000351	200	216	PR01013	Neuropeptide Y1 receptor
	IPR000276	220	243	PR00237	Rhodopsin-like GPCR superfamily
HMMPfam	IPR000276	65	261	PF00001	Rhodopsin-like GPCR superfamily
ProfileScan	IPR000276	65	242	PS50262	Rhodopsin-like GPCR superfamily
ScanRegExp	IPR000276	134	150	PS00237	Rhodopsin-like GPCR superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	47	MIFTLALAYGAVIILGVSGNLAL	69	PRIMARY	23
	2	85	ILIVNLSFSDLLVAIMCLPFTFV	107	PRIMARY	23
	3	126	FVQCVSITVSIFSLVLIAVERH	147	PRIMARY	22
	4	165	VGIAVIWVLAVASSLPFLIYQVM	187	PRIMARY	23
	5	219	YTTLLLVLQYFGPLCFIFICYFK	241	PRIMARY	23
	6	245	LFFYHFHFYLLYTEFLIK	262	SECONDARY	18