ORK06661

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06661

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Accession No	AB209800
Product ID	ORK06661
Clone name	bm03528
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	RNF130
cDNA sequence	DNA sequence (1734 bp) Predicted protein sequence (401 aa)
Description	Goliath homolog precursor (RING finger protein 130).

Features of the cloned cDNA sequence

Description

Length: 1734 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	180 bp
Genome contig ID	gi51511721r_179215091
PolyA signal sequence (AATAAA,-8)	+--------+--------+----*----+---- CAAAGCTTTGTATTAAAAGAAATAAATAATAAAAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAGTCTGTGCTGTTGAGTCTTATTAACCAGACCTGTGTTTTGAGGGC

Features of the protein sequence

Description

Length: 401 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD93037	5.4e-166	100.0	ring finger pro...	Homo sapiens
XP_001143540	1.3e-165	99.7	ring finger pro...	Pan troglodytes
EAW53773	2e-158	100.0	ring finger pro...	Homo sapiens
Q86XS8	3.8e-158	99.7	Goliath homolog...	Homo sapiens
AAH17100	1.1e-157	99.7	RNF130 protein ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003137	88	186	PF02225	Protease-associated PA
HMMPfam	IPR001841	281	321	PF00097	Zinc finger
HMMSmart	IPR001841	281	321	SM00184	Zinc finger
ProfileScan	IPR001841	281	322	PS50089	Zinc finger

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	23	RAGPARLAALALLTCSLWPAR	43	SECONDARY	21
	2	180	KDILSYLEKNISVQMTIAVGTRM	202	SECONDARY	23
	3	212	LVFVSISFIVLMIISSAWLIFYF	234	PRIMARY	23