ORK06849

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06849

Order Kazusa clone(s) from :

Accession No	AB209199
Product ID	ORK06849
Clone name	fj04618
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	SLC1A6
cDNA sequence	DNA sequence (4070 bp) Predicted protein sequence (205 aa)
Description	Excitatory amino acid transporter 4 (Solute carrier family 1 member 6) (Sodium-dependent glutamate/aspartate transporter).

Features of the cloned cDNA sequence

Description

Length: 4070 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	286 bp
Genome contig ID	gi42406306r_14835939
PolyA signal sequence (None)	+--------+--------+----*----+---- CAGCCTGGGCAACAGAATGAGACTCTCTGTCTTTC
Flanking genome sequence (99882 - 99833)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAGATTAAAAAAAAATTAGCTGGACACGCCTGTCTCAGC

Features of the protein sequence

Description

Length: 205 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92436	5.5e-82	100.0	solute carrier ...	Homo sapiens
XP_001111555	4.3e-81	99.5	solute carrier ...	Macaca mulatta
AAH28721	2.7e-79	97.5	SLC1A6 protein ...	Homo sapiens
P48664	4.1e-79	97.5	Excitatory amin...	Homo sapiens
BAE00642	1.5e-78	97.0	unnamed protein...	Macaca fascicularis

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001991	57	77	PR00173	Sodium:dicarboxylate symporter
	IPR001991	100	120	PR00173	Sodium:dicarboxylate symporter
	IPR001991	129	154	PR00173	Sodium:dicarboxylate symporter
HMMPfam	IPR001991	59	199	PF00375	Sodium:dicarboxylate symporter
ScanRegExp	IPR001991	93	107	PS00713	Sodium:dicarboxylate symporter

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	57	NAFILLTVSAVVIGVSLAFALRP	79	SECONDARY	23
	2	98	MRMLQMLVLPLIVSSLVTGMASL	120	PRIMARY	23
	3	132	AAVYYMVTTIIAVFIGILMVTII	154	PRIMARY	23