ORK06888

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06888

Order Kazusa clone(s) from :

Accession No	AB209704
Product ID	ORK06888
Clone name	bm00750
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	SLC6A8
cDNA sequence	DNA sequence (2149 bp) Predicted protein sequence (644 aa)
Description	Sodium- and chloride-dependent creatine transporter 1 (CT1) (Creatine transporter 1) (Solute carrier family 6 member 8).

Features of the cloned cDNA sequence

Description

Length: 2149 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	189 bp
Genome contig ID	gi89161218f_152507206
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- ACTTTTTCCATTTTTAATAAAACGCCAAAAATATC
Flanking genome sequence (106848 - 106897)	----+--------+--------+--------+--------+----* ACAACCCACCAAAAATAGATGCCTCTCCCCCTCCAGCCCTAGCCGAGCTG

Features of the protein sequence

Description

Length: 644 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92941	0	100.0	solute carrier ...	Homo sapiens
CAM21057	1.1e-202	96.6	solute carrier ...	Mus musculus
AAH49801	1.4e-180	97.0	Slc6a8 protein ...	Mus musculus
EAW72829	9e-133	97.3	hCG2007960, iso...	Homo sapiens
EAW72827	9.6e-133	97.4	hCG2007960, iso...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000175	70	98	PD000448	Sodium:neurotransmitter symporter
FPrintScan	IPR002984	20	33	PR01199	Creatine transporter
	IPR000175	79	100	PR00176	Sodium:neurotransmitter symporter
	IPR000175	108	127	PR00176	Sodium:neurotransmitter symporter
	IPR000175	151	177	PR00176	Sodium:neurotransmitter symporter
	IPR002984	200	211	PR01199	Creatine transporter
	IPR002984	213	226	PR01199	Creatine transporter
	IPR000175	279	296	PR00176	Sodium:neurotransmitter symporter
	IPR000175	405	424	PR00176	Sodium:neurotransmitter symporter
	IPR000175	489	509	PR00176	Sodium:neurotransmitter symporter
	IPR000175	529	549	PR00176	Sodium:neurotransmitter symporter
HMMPfam	IPR000175	71	606	PF00209	Sodium:neurotransmitter symporter
ProfileScan	IPR000175	70	594	PS50267	Sodium:neurotransmitter symporter
ScanRegExp	IPR000175	95	109	PS00610	Sodium:neurotransmitter symporter
ScanRegExp	IPR000175	177	197	PS00754	Sodium:neurotransmitter symporter

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	77	QMDFIMSCVGFAVGLGNVWRFPY	99	SECONDARY	23
	2	108	VFLIPYVLIALVGGIPIFFLEIS	130	PRIMARY	23
	3	149	FKGLGYASMVIVFYCNTYYIMVL	171	PRIMARY	23
	4	251	NWEVTLCLLACWVLVYFCVWKGV	273	PRIMARY	23
	5	287	PYVVLVVLLVRGVLLPGALDGII	309	PRIMARY	23
	6	327	DAGTQIFFSYAIGLGALTALGSY	349	SECONDARY	23
	7	358	NGTSFFAGFVVFSILGFMAAEQG	380	SECONDARY	23
	8	395	FIAYPRAVTLMPVAPLWAALFFF	417	PRIMARY	23
	9	451	QREISVALCCALCFVIDLSMVTD	473	PRIMARY	23
	10	499	ECVVVAWVYGADRFMDDIACMIG	521	SECONDARY	23
	11	533	SFFTPLVCMGIFIFNVVYYEPLV	555	PRIMARY	23
	12	570	GWAFALSSMLCVPLHLLGCLLR	591	SECONDARY	22