ORK07190

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK07190

Order Kazusa clone(s) from :

Accession No	AB209253
Product ID	ORK07190
Clone name	fj20184
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	TPCN2
cDNA sequence	DNA sequence (4376 bp) Predicted protein sequence (458 aa)
Description	Two-pore calcium channel protein 2 (Voltage-dependent calcium channel protein TPC2).

Features of the cloned cDNA sequence

Description

Length: 4376 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	2997 bp
Genome contig ID	gi51511727f_68479206
PolyA signal sequence (AATAAA,-33)	+--------+--------+----*----+---- GCAATAAATATGTAAGTACATAAAAACAGCAATAG
Flanking genome sequence (135444 - 135493)	----+--------+--------+--------+--------+----* AAGAAACGTAATGCTTTATTCTCAAATATGATGTCTACATAGAAAAGCCA

Features of the protein sequence

Description

Length: 458 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92490	1e-197	100.0	two pore segmen...	Homo sapiens
AAK31802	1e-107	68.6	two-pore calciu...	Homo sapiens
Q8NHX9	1e-107	68.4	Two pore calciu...	Homo sapiens
BAG61025	1.8e-107	96.7	unnamed protein...	Homo sapiens
XP_001101571	1.8e-105	67.0	similar to two ...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005821	47	231	PF00520	Ion transport

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	3	SRTLSFTIFLILFLAFIETPSSL	25	PRIMARY	23
	2	42	CGLTESVEVLCLLVFAADLSVKG	64	PRIMARY	23
	3	79	LGYLVVLVVSLVDWTVSLSLVCH	101	PRIMARY	23
	4	138	SVGLLLAIHLCLFTMFGMLLFA	159	PRIMARY	22
	5	208	YAIFFIVFTVIGSLFLMNLLTAI	230	PRIMARY	23
	6	311	CPFACSWCWMQMCCLLSVMTSSW	333	SECONDARY	23