ORK07547

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK07547

Order Kazusa clone(s) from :

Accession No	AB209752
Product ID	ORK07547
Clone name	bg00122
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	SLC4A5
cDNA sequence	DNA sequence (6571 bp) Predicted protein sequence (906 aa)
Description	sodium bicarbonate transporter 4 isoform c

Features of the cloned cDNA sequence

Description

Length: 6571 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	2784 bp
Genome contig ID	gi89161199r_74196878
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- AACTGTAATTCAATAAAATTTAATCCTGTCTGGTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATCTTGCTCCACATTTAATAATCTAGGTAAGCAGGCATGGATTAGAGACA

Features of the protein sequence

Description

Length: 906 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92989	100.0	sodium bicarbon...	Homo sapiens
XP_001154731	99.7	sodium bicarbon...	Pan troglodytes
AAK97073	99.8	sodium bicarbon...	Homo sapiens
AAG18492	83.6	sodium bicarbon...	Homo sapiens
AAK97072	99.8	sodium bicarbon...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003024	55	61	PR01232	Na+/HCO3- transporter
	IPR003020	251	263	PR01231	HCO3- transporter
	IPR003024	261	269	PR01232	Na+/HCO3- transporter
	IPR003024	302	310	PR01232	Na+/HCO3- transporter
	IPR003024	312	320	PR01232	Na+/HCO3- transporter
	IPR003024	322	334	PR01232	Na+/HCO3- transporter
	IPR003020	539	551	PR01231	HCO3- transporter
	IPR003020	560	572	PR01231	HCO3- transporter
	IPR003020	693	702	PR01231	HCO3- transporter
	IPR003020	757	766	PR01231	HCO3- transporter
HMMPfam	IPR013769	54	321	PF07565	HCO3- transporter
HMMPfam	IPR011531	400	778	PF00955	HCO3-transporter
HMMTigr	IPR003020	27	900	TIGR00834	HCO3- transporter

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	434	SISAILFIYLGCITNAITFGGLL	456	SECONDARY	23
	2	476	AGSLFCLFSGQPLIILSSTGPIL	498	SECONDARY	23
	3	524	LHSAVQCLILVATDASFIIKYIT	546	PRIMARY	23
	4	552	GFSTLISFIFIYDAIK	567	SECONDARY	16
	5	649	SCKFIPDLALMSFILFFGTYSMT	671	SECONDARY	23
	6	688	RALVADFSIVFSILMFCGIDACF	710	PRIMARY	23
	7	743	WVYPASILPALLVTILIFMDQQI	765	PRIMARY	23