ORK10385

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK10385

Order Kazusa clone(s) from :

Accession No
Product ID	ORK10385
Clone name	bm04855
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	CDIPT
cDNA sequence	DNA sequence (1817 bp) Predicted protein sequence (287 aa)
HaloTag ORF Clone	FHC10385
Flexi ORF Clone	FXC10385
Description	CDP-diacylglycerol--inositol 3-phosphatidyltransferase (EC 2.7.8.11)(Phosphatidylinositol synthase)(PtdIns synthase)(PI synthase)

Features of the cloned cDNA sequence

Description

Length: 1817 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	827 bp
Genome contig ID	gi51511732r_29677327
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- GCATATAGTAGCTGCTTAATAAATACTCGTCTGTC
Flanking genome sequence (99857 - 99808)	----+--------+--------+--------+--------+----* AATGAGTTGGTTTCTCAGAGTGTGGTCCTTAGACCACCTGTGGTATATTT

Features of the protein sequence

Description

Length: 287 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
O14735	1.9e-79	100.0	CDP-diacylglyce...	Homo sapiens
AAP36554	1.9e-79	100.0	CDP-diacylglyce...	synthetic construct
AAX37049	1.9e-79	100.0	CDP-diacylglyce...	synthetic construct
BAD96396	7.8e-79	99.5	CDP-diacylglyce...	Homo sapiens
XP_001105279	1.4e-78	99.0	CDP-diacylglyce...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000462	111	271	PF01066	CDP-alcohol phosphatidyltransferase
ScanRegExp	IPR000462	124	146	PS00379	CDP-alcohol phosphatidyltransferase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	91	ARIVFAIISFYFMPCCPLTASSF	113	PRIMARY	23
	2	151	MCLLVNLALLYPGATLFFQISMS	173	SECONDARY	23
	3	210	TSRPALFTLCAGNELFYCLLYLF	232	SECONDARY	23
	4	244	GLFRMGLWVTAPIALLKSLISVI	266	PRIMARY	23