ORK10386

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK10386

Order Kazusa clone(s) from :

Accession No
Product ID	ORK10386
Clone name	bm05106
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	AGPAT4
cDNA sequence	DNA sequence (1739 bp) Predicted protein sequence (383 aa)
HaloTag ORF Clone	FHC10386
Flexi ORF Clone	FXC10386
Description	1-acyl-sn-glycerol-3-phosphate acyltransferase delta (EC 2.3.1.51)(1-AGP acyltransferase 4)(1-AGPAT 4)(Lysophosphatidic acid acyltransferase delta)(LPAAT-delta)(1-acylglycerol-3-phosphate O-acyltransferase 4)

Features of the cloned cDNA sequence

Description

Length: 1739 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	464 bp
Genome contig ID	gi89161210r_161377098
PolyA signal sequence (ATTAAA,-20)	+--------+--------+----*----+---- CTCCAAGAAAAAAAAATTAAAGTGCTTTTCTGGGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* CATCTGTGGTGTATGTTGACATGGACCGCTGCCCCTCCATCCTGCCCTTG

Features of the protein sequence

Description

Length: 383 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9NRZ5	3e-173	100.0	1-acyl-sn-glyce...	Homo sapiens
Q5R757	1.3e-172	99.7	1-acyl-sn-glyce...	Pongo abelii
Q4R581	1e-170	98.4	1-acyl-sn-glyce...	Macaca fascicularis
XP_001500501	4.6e-163	93.1	similar to 1-ac...	Equus caballus
XP_001153359	5.4e-163	94.4	1-acylglycerol-...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002123	92	232	PF01553	Phospholipid/glycerol acyltransferase
HMMSmart	IPR002123	95	217	SM00563	Phospholipid/glycerol acyltransferase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	6	MDLAGLLKSQFLCHLVFCYVFIA	28	SECONDARY	23
	2	313	LVNWLFWASLVLYPFFQFLVSMI	335	PRIMARY	23
	3	343	LASFILVFFVASVGVRWMIGVTE	365	PRIMARY	23