ORK11455

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11455

Order Kazusa clone(s) from :

Accession No
Product ID	ORK11455
Clone name	ha02351
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of th ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
Symbol	SCD
cDNA sequence	DNA sequence (2214 bp) Predicted protein sequence (366 aa)
HaloTag ORF Clone	FHC11455
Flexi ORF Clone	FXC11455
Description	carnitine deficiency, systemic primary

Features of the cloned cDNA sequence

Description

Length: 2214 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	887 bp
Genome contig ID	gi89161187f_101997005
PolyA signal sequence (None)	+--------+--------+----*----+---- ATATATCAAGAAGTCTTGAAGTTGGGTGTTTCCAG
Flanking genome sequence (114564 - 114613)	----+--------+--------+--------+--------+----* AATTGGTAAAAACAGCAGCTCATAGAATTTTGAGTATTCCATGAGCTGCT

Features of the protein sequence

Description

Length: 366 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92219	3.8e-167	100.0	stearoyl-CoA de...	Homo sapiens
AAD29870	1.2e-163	100.0	stearoyl-CoA de...	Homo sapiens
O00767	1.6e-163	99.7	Acyl-CoA desatu...	Homo sapiens
BAD96582	1.3e-162	99.4	stearoyl-CoA de...	Homo sapiens
CAH91238	1.5e-162	99.1	hypothetical pr...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR010257	105	188	PD001081	Fatty acid desaturase
BlastProDom	IPR001522	232	366	PD002221	Fatty acid desaturase
FPrintScan	IPR015876	80	100	PR00075	Fatty acid desaturase
	IPR015876	104	126	PR00075	Fatty acid desaturase
	IPR015876	127	147	PR00075	Fatty acid desaturase
	IPR015876	164	193	PR00075	Fatty acid desaturase
	IPR015876	225	243	PR00075	Fatty acid desaturase
	IPR015876	258	279	PR00075	Fatty acid desaturase
	IPR015876	301	315	PR00075	Fatty acid desaturase
HMMPfam	IPR005804	103	328	PF00487	Fatty acid desaturase
ScanRegExp	IPR001522	301	315	PS00476	Fatty acid desaturase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	80	WRNIILMSLLHLGALYGITLIPT	102	PRIMARY	23
	2	107	TWLWGVFYYFVSALGITAGAHRL	129	SECONDARY	23
	3	138	RLPLRLFLIIANTMAFQNDVYEW	160	SECONDARY	23
	4	225	YKPGLLLMCFILPTLVPWYFWGE	247	PRIMARY	23
	5	253	VFVATFLRYAVVLNATWLVNSAA	275	SECONDARY	23
	6	323	HINFTTFFIDCMAALGLAYD	342	SECONDARY	20