ORK11832

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK11832

Order Kazusa clone(s) from :

Accession No
Product ID	ORK11832
Clone name	hh03515
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRIA1
cDNA sequence	DNA sequence (5454 bp) Predicted protein sequence (919 aa)
Description	Glutamate receptor 1 Precursor (GluR-1)(GluR-A)(GluR-K1)(Glutamate receptor ionotropic, AMPA 1)(AMPA-selective glutamate receptor 1)

Features of the cloned cDNA sequence

Description

Length: 5454 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2454 bp
Genome contig ID	gi51511721f_152750363
PolyA signal sequence (ATTAAA,-20)	+--------+--------+----*----+---- ACAGTTCTGGCTTTCATTAAATTTTGCTCTTTGGT
Flanking genome sequence (423071 - 423120)	----+--------+--------+--------+--------+----* ACCTGGGCCTTTTATTTAACATCTATATTTGTTTTAACTCTCTTGGCAGA

Features of the protein sequence

Description

Length: 919 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW61651	100.0	glutamate recep...	Homo sapiens
BAF82254	99.7	unnamed protein...	Homo sapiens
XP_001503669	99.8	glutamate recep...	Equus caballus
XP_546286	99.7	similar to Glut...	Canis lupus fam...
AAA58613	99.5	glutamate recep...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	621	687	PD245531	NULL
FPrintScan	IPR001508	465	493	PR00177	NMDA receptor
	IPR001508	627	654	PR00177	NMDA receptor
	IPR001508	819	843	PR00177	NMDA receptor
HMMPfam	IPR001828	50	386	PF01094	Extracellular ligand-binding receptor
HMMPfam	IPR001320	549	840	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	421	796	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	14	MQHIFAFFCTGFLGAVVGANFPN	36	SECONDARY	23
	2	546	DPLAYEIWMCIVFAYIGVSVVLF	568	PRIMARY	23
	3	597	EFGIFNSLWFSLGAFMQQGCDIS	619	SECONDARY	23
	4	632	WWFFTLIIISSYTANLAAFLTVE	654	PRIMARY	23
	5	817	SNVAGVFYILIGGLGLAMLVALI	839	PRIMARY	23