ORK01802

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01802

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01802
Clone name	bm00984
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	S1PR5
cDNA sequence	DNA sequence (2118 bp) Predicted protein sequence (415 aa)
Description	Sphingosine 1-phosphate receptor Edg-8 (Endothelial differentiation sphingolipid G-protein-coupled receptor 8) (Sphingosine 1-phosphate receptor 5) (S1P5).

Features of the cloned cDNA sequence

Description

Length: 2118 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	869 bp
Genome contig ID	gi42406306r_10384635
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- TCCTATCTGGTAATAAAATTTCTTTTTTATTTTTC
Flanking genome sequence (99987 - 99938)	----+--------+--------+--------+--------+----* TTTTCTTTCTTTCTTTCTTTTTTTTTTTTTTGAGTTGGGATCTTGTGCTC

Features of the protein sequence

Description

Length: 415 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EAW84113	2.8e-158	100.0	endothelial dif...	Homo sapiens
Q9H228	1.9e-150	100.0	Sphingosine 1-p...	Homo sapiens
ABM82216	3.9e-150	99.7	endothelial dif...	synthetic construct
XP_001101402	3.7e-142	95.2	similar to endo...	Macaca mulatta
Q684M3	4.9e-128	86.6	Sphingosine 1-p...	Sus scrofa

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR005386	18	33	PR01561	EDG-8 sphingosine 1-phosphate receptor
	IPR004061	27	39	PR01523	Sphingosine 1-phosphate receptor
	IPR005386	42	57	PR01561	EDG-8 sphingosine 1-phosphate receptor
	IPR000276	55	79	PR00237	Rhodopsin-like GPCR superfamily
	IPR005386	79	89	PR01561	EDG-8 sphingosine 1-phosphate receptor
	IPR000276	88	109	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	106	115	PR01523	Sphingosine 1-phosphate receptor
	IPR004061	126	137	PR01523	Sphingosine 1-phosphate receptor
	IPR000276	132	154	PR00237	Rhodopsin-like GPCR superfamily
	IPR005386	157	171	PR01561	EDG-8 sphingosine 1-phosphate receptor
	IPR000276	167	188	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	208	218	PR01523	Sphingosine 1-phosphate receptor
	IPR000276	210	233	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	229	238	PR01523	Sphingosine 1-phosphate receptor
	IPR005386	237	249	PR01561	EDG-8 sphingosine 1-phosphate receptor
	IPR005386	251	261	PR01561	EDG-8 sphingosine 1-phosphate receptor
	IPR000276	266	290	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	297	308	PR01523	Sphingosine 1-phosphate receptor
	IPR000276	305	331	PR00237	Rhodopsin-like GPCR superfamily
	IPR004061	326	336	PR01523	Sphingosine 1-phosphate receptor
	IPR005386	341	352	PR01561	EDG-8 sphingosine 1-phosphate receptor
	IPR005386	353	364	PR01561	EDG-8 sphingosine 1-phosphate receptor
	IPR005386	365	387	PR01561	EDG-8 sphingosine 1-phosphate receptor
	IPR005386	388	409	PR01561	EDG-8 sphingosine 1-phosphate receptor
HMMPfam	IPR000276	70	323	PF00001	Rhodopsin-like GPCR superfamily
ProfileScan	IPR000276	70	323	PS50262	Rhodopsin-like GPCR superfamily
ScanRegExp	IPR000276	138	154	PS00237	Rhodopsin-like GPCR superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	55	ADAVVCLAVCAFIVLENLAVLLV	77	PRIMARY	23
	2	94	SLTLSDLLAGAAYAANILLSGPL	116	SECONDARY	23
	3	131	GVFVALTASVLSLLAIALERSLT	153	PRIMARY	23
	4	169	LAMAAAAWGVSLLLGLLPALGWN	191	PRIMARY	23
	5	207	AKAYVLFCVLAFVGILAAICALY	229	PRIMARY	23
	6	268	RTLSVVLLAFVACWGPLFLLLLL	290	PRIMARY	23
	7	306	DPFLGLAMANSLLNPIIYTLTNR	328	SECONDARY	23