ORK05250

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05250

Order Kazusa clone(s) from :

Accession No	AB209734
Product ID	ORK05250
Clone name	bm04359
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	FZD6
cDNA sequence	DNA sequence (2095 bp) Predicted protein sequence (328 aa)
Description	Frizzled-6 precursor (Fz-6) (hFz6).

Features of the cloned cDNA sequence

Description

Length: 2095 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1106 bp
Genome contig ID	gi51511724f_104306004
PolyA signal sequence (None)	+--------+--------+----*----+---- AGTAAAAAAAAATTTTTTTAAAGATTCTGCGTGAC
Flanking genome sequence (102096 - 102145)	----+--------+--------+--------+--------+----* AAAAAAAAACAAACAAACAATCCATAATCAATGTCAAAAGACTAACTGGG

Features of the protein sequence

Description

Length: 328 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92971	5.6e-149	100.0	Putative G-prot...	Homo sapiens
BAB89325	4.3e-137	99.6	putative G-prot...	Homo sapiens
EAW91872	2.5e-135	91.9	frizzled homolo...	Homo sapiens
O60353	4.1e-135	91.9	Frizzled-6; Sho...	Homo sapiens
XP_519897	5.3e-135	91.6	hypothetical pr...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000539	36	58	PR00489	Frizzled protein
	IPR000539	64	86	PR00489	Frizzled protein
	IPR000539	119	143	PR00489	Frizzled protein
	IPR000539	162	185	PR00489	Frizzled protein
	IPR000539	207	229	PR00489	Frizzled protein
	IPR000539	248	269	PR00489	Frizzled protein
HMMPfam	IPR000539	23	328	PF01534	Frizzled protein
ProfileScan	IPR000832	30	264	PS50261	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	35	SFIGTVSIFCLCATLFTFLTFLI	57	PRIMARY	23
	2	70	IYYSVCYSIVSLMYFIGFLLGDS	92	SECONDARY	23
	3	120	LFMLLYFFTMAGTVWWVILTITW	142	PRIMARY	23
	4	160	VWFHAVAWGTPGFLTVMLLALNK	182	SECONDARY	23
	5	206	VLLPLCLCVFVGLSLLLAGIISL	228	PRIMARY	23
	6	249	MIRIGVFSGLYLVPLVTLLGCYV	271	PRIMARY	23
	7	298	YQVKAITWIMSLFTFNVENVSWK	320	SECONDARY	23