ORK06850

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06850

Order Kazusa clone(s) from :

Accession No	AB209484
Product ID	ORK06850
Clone name	ff01216
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	SLC22A5
cDNA sequence	DNA sequence (10439 bp) Predicted protein sequence (440 aa)
Description	Organic cation/carnitine transporter 2 (Solute carrier family 22 member 5) (High-affinity sodium-dependent carnitine cotransporter).

Features of the cloned cDNA sequence

Description

Length: 10439 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1341 bp
Genome contig ID	gi51511721f_131639677
PolyA signal sequence (AGTAAA,-23)	+--------+--------+----*----+---- AAAGTTTTAATAAGTAAAATGATTTTTTAAATATC
Flanking genome sequence (119529 - 119578)	----+--------+--------+--------+--------+----* AGCAACTGCCTAGAATCTTTATTTCTAGAATTTAGTGAGCCTGTGCCACA

Features of the protein sequence

Description

Length: 440 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92721	3.9e-173	100.0	solute carrier ...	Homo sapiens
AAH12325	4.2e-150	90.6	Solute carrier ...	Homo sapiens
EAW62339	6.4e-150	91.4	solute carrier ...	Homo sapiens
O76082	1.1e-149	95.4	Solute carrier ...	Homo sapiens
BAF45812	1.2e-149	95.4	organic cation ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR011701	49	369	PF07690	Major facilitator superfamily MFS_1
ProfileScan	IPR007114	1	397	PS50850	Major facilitator superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	54	NVLFVTMGMQTGFSFLQIFSKNF	76	SECONDARY	23
	2	78	MFVVLFVLVGMGQISNYVAAFVL	100	PRIMARY	23
	3	111	IIFSTLGVCIFYAFGYMVLPLFA	133	PRIMARY	23
	4	140	RMLLVALTMPGVLCVALWWFIPE	162	PRIMARY	23
	5	224	RMVTIMSIMLWMTISVGYFGLSL	246	PRIMARY	23
	6	256	FVNCFLSAMVEVPAYVLAWLLLQ	278	SECONDARY	23
	7	284	YSMATALFLGGSVLLFMQLVPPD	306	PRIMARY	23
	8	309	YLATVLVMVGKFGVTAAFSMVYV	331	PRIMARY	23
	9	374	PYILMGSLTILTAILTLFLPESF	396	PRIMARY	23