ORK04414

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK04414

Order Kazusa clone(s) from :

Accession No	AB209499
Product ID	ORK04414
Clone name	ff07566
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	CACNA1E
cDNA sequence	DNA sequence (11702 bp) Predicted protein sequence (1869 aa)
Description	Voltage-dependent R-type calcium channel subunit alpha-1E (Voltage- gated calcium channel subunit alpha Cav2.3) (Calcium channel, L type, alpha-1 polypeptide, isoform 6) (Brain calcium channel II) (BII).

Features of the cloned cDNA sequence

Description

Length: 11702 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	6092 bp
Genome contig ID	gi89161185f_179851797
PolyA signal sequence (None)	+--------+--------+----*----+---- CATTTTCCCTTATTCTTCTTGTTGTCTCTCTAATT
Flanking genome sequence (188888 - 188937)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAACCTAGGCTTTTTTTTTTCTATCCAGAAAGTTGGGTT

Features of the protein sequence

Description

Length: 1869 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92736	100.0	calcium channel...	Homo sapiens
CAH72675	99.9	calcium channel...	Homo sapiens
EAW91109	99.7	calcium channel...	Homo sapiens
AAA59205	99.6	voltage-depende...	Homo sapiens
XP_001914915	96.4	similar to calc...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR005449	58	70	PR01633	R-type voltage-dependent calcium channel alpha 1 subunit
	IPR002077	192	207	PR00167	Ca2+ channel
	IPR005449	486	499	PR01633	R-type voltage-dependent calcium channel alpha 1 subunit
	IPR005449	529	539	PR01633	R-type voltage-dependent calcium channel alpha 1 subunit
	IPR005449	663	682	PR01633	R-type voltage-dependent calcium channel alpha 1 subunit
	IPR002077	1023	1043	PR00167	Ca2+ channel
	IPR002077	1195	1209	PR00167	Ca2+ channel
	IPR002077	1243	1255	PR00167	Ca2+ channel
HMMPfam	IPR005821	109	301	PF00520	Ion transport
	IPR005821	787	1021	PF00520	Ion transport
	IPR005821	1111	1321	PF00520	Ion transport
	IPR014873	1459	1492	PF08763	Voltage gated calcium channel IQ domain
ProfileScan	IPR002048	1338	1373	PS50222	Calcium-binding EF-hand

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	75	QVFYWIVLSLVALNTACVAIVH	96	PRIMARY	22
	2	105	HLLYYAEFLFLGLFLLEMSLKMY	127	PRIMARY	23
	3	140	NCFDFGVTVGSIFEVVWAIFRPG	162	SECONDARY	23
	4	194	SLMSSMKSIISLLFLLFLFIVVF	216	PRIMARY	23
	5	281	YFIVLTLFGNYTLLNVFLAIAVD	303	PRIMARY	23
	6	753	YFEMCILLVIAASSIALAAEDPV	775	PRIMARY	23
	7	820	LWNILDFVVVVGALVAFALANAL	842	SECONDARY	23
	8	885	LKNVFNILIVYKLFMFIFAVIAV	907	PRIMARY	23
	9	952	FHYDNIIWALLTLFTVSTGEGWP	974	SECONDARY	23
	10	1001	FYVVYFVVFPFFFVNIFVALIII	1023	PRIMARY	23
	11	1077	PSFEYTIMAMIALNTVVLMMKY	1098	PRIMARY	22
	12	1112	LNIAFTMVFSLECVLKVIAFGFL	1134	PRIMARY	23
	13	1179	AARLIKLLRQGYTIRILLWTFVQ	1201	SECONDARY	23
	14	1208	YVCLLIAMLFFIYAIIGMQVFGN	1230	PRIMARY	23
	15	1301	YFVSFIFFCSFLMLNLFVAVIMD	1323	PRIMARY	23