ORK10405

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK10405

Order Kazusa clone(s) from :

Accession No
Product ID	ORK10405
Clone name	fh03725
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	LIME1
cDNA sequence	DNA sequence (5196 bp) Predicted protein sequence (433 aa)
Description	Lck-interacting transmembrane adapter 1 (Lck-interacting membrane protein)(Lck-interacting molecule)

Features of the cloned cDNA sequence

Description

Length: 5196 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	203 bp
Genome contig ID	gi51511747f_61710403
PolyA signal sequence (AATAAA,-28)	+--------+--------+----*----+---- GCGTCTAAATAAAGCGCCAGCGCAGGATGAAAGCG
Flanking genome sequence (130499 - 130548)	----+--------+--------+--------+--------+----* GCCAGCCTCGCAGCCTGCTCTTCTTGAAAGCTGGGCGGGTTGGGGCGGGG

Features of the protein sequence

Description

Length: 433 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9H400	6.2e-109	100.0	Lck-interacting...	Homo sapiens
BAA91148	2.1e-108	99.6	unnamed protein...	Homo sapiens
AAP34480	5.6e-71	100.0	LP8067 [Homo sa...	Homo sapiens
XP_001113808	3e-64	76.2	similar to zinc...	Macaca mulatta
CAI21882	3.9e-35	100.0	novel protein [...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	28	LVTIWAMVATAVLPLLTAVLGVT	50	PRIMARY	23
SOSUI2	2	148	PALWVLGCCALLLSLWALCTAC	169	PRIMARY	22