ORK07582

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK07582

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Accession No	AB209356
Product ID	ORK07582
Clone name	fh15402
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	FADS3
cDNA sequence	DNA sequence (5322 bp) Predicted protein sequence (315 aa)
Description	fatty acid desaturase 3

Features of the cloned cDNA sequence

Description

Length: 5322 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	2009 bp
Genome contig ID	gi51511727r_61297572
PolyA signal sequence (ATTAAA,-23)	+--------+--------+----*----+---- TGTTATTTTTATATTAAAATACATTCAGATGTATT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATGGAGTGGAGTGGTGAATTTGCATGAGTTTTGAAGCTGGGGTCTGCCCA

Features of the protein sequence

Description

Length: 315 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92593	2.3e-142	100.0	fatty acid desa...	Homo sapiens
AAC23396	2.4e-86	97.0	BC269730_1 [Hom...	Homo sapiens
Q9Y5Q0	2.9e-86	97.0	Fatty acid desa...	Homo sapiens
XP_001150669	2.9e-86	97.0	fatty acid desa...	Pan troglodytes
XP_533270	7.7e-78	86.4	similar to fatt...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001199	7	37	PD000612	Cytochrome b5
BlastProDom	IPR010257	76	169	PD001081	Fatty acid desaturase
HMMPfam	IPR005804	102	295	PF00487	Fatty acid desaturase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	65	ASPTFFAFLLGHILAMEVLAWLL	87	PRIMARY	23
SOSUI2	2	93	PGWVPSALAAFILAISQAQSWCL	115	SECONDARY	23