ORK05553

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05553

Order Kazusa clone(s) from :

Accession No	AB208868
Product ID	ORK05553
Clone name	hg04036
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector. This cDNA clone hg04036 has an irregular adapter sequence (GTCGACCCAC GCGTCCGCCC ACGCGTCCG) instead of regular one (GTCGACCCAC GCGTCCG).
Symbol	ITPR1
cDNA sequence	DNA sequence (6524 bp) Predicted protein sequence (1207 aa)
Description	Inositol 1,4,5-trisphosphate receptor type 1 (Type 1 inositol 1,4,5- trisphosphate receptor) (Type 1 InsP3 receptor) (IP3 receptor isoform 1) (InsP3R1) (IP3R).

Features of the cloned cDNA sequence

Description

Length: 6524 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	1170 bp
Genome contig ID	gi89161205f_4593441
PolyA signal sequence (None)	+--------+--------+----*----+---- TACATGGATCTCATTTTACAAGAGAATCTCTCTGC
Flanking genome sequence (270640 - 270689)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAACAGTTTAAAAATGCATTGAAAGCAGAGTTCTGAAAT

Features of the protein sequence

Description

Length: 1207 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92105	100.0	Inositol 1,4,5-...	Homo sapiens
Q14643	99.7	Inositol 1,4,5-...	Homo sapiens
XP_862809	97.7	similar to Inos...	Canis lupus fam...
XP_862834	97.7	similar to Inos...	Canis lupus fam...
XP_862857	97.7	similar to Inos...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013662	1	533	PF08454	RyR and IP3R Homology associated
HMMPfam	IPR005821	820	1046	PF00520	Ion transport

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	733	WSSISFNLAVLMNLLVAFFYPFK	755	PRIMARY	23
	2	775	LISLAIVIALPKPHGIRALIAST	797	PRIMARY	23
	3	814	LGAFNVCNKIIFLMSFVGNCGTF	836	SECONDARY	23
	4	849	FLYHLLYLVICAMGLFVHEFFYS	871	PRIMARY	23
	5	897	SIILTAVLALILVYLFSIVGYLF	919	PRIMARY	23
	6	1024	VIYDLLFFFMVIIIVLNLIFGVI	1046	PRIMARY	23