ORK00117

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK00117

Order Kazusa clone(s) from :

Accession No
Product ID	ORK00117
Clone name	hh12762
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	SNX13
cDNA sequence	DNA sequence (6360 bp) Predicted protein sequence (1024 aa)
HaloTag ORF Clone	FHC00117
Flexi ORF Clone	FXC00117
Description	Sorting nexin-13 (RGS domain- and PHOX domain-containing protein) (RGS-PX1).

Features of the cloned cDNA sequence

Description

Length: 6360 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	3283 bp
Genome contig ID	gi89161213r_17696911
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- GTCCATTAGAAAATACTAATAAAGTATTAACAAAC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTTTTGTTGAGTTCATTTAATAGTAATGTATTTTTGTTACTTTTAAGAT

Features of the protein sequence

Description

Length: 1024 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_518985	99.2	similar to RGS-...	Pan troglodytes
XP_001252605	96.7	hypothetical pr...	Bos taurus
EDL36865	96.3	sorting nexin 1...	Mus musculus
AAL37728	100.0	RGS-PX1 [Homo s...	Homo sapiens
BAG57773	99.7	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000342	496	580	PD001580	Regulator of G protein signalling
HMMPfam	IPR003114	164	351	PF02194	PX-associated
	IPR000342	507	563	PF00615	Regulator of G protein signalling
	IPR001683	630	743	PF00787	Phox-like
	IPR013937	859	970	PF08628	Sorting nexin
HMMSmart	IPR013996	164	351	SM00313	PX-associated
	IPR000342	440	580	SM00315	Regulator of G protein signalling
	IPR001683	630	743	SM00312	Phox-like
ProfileScan	IPR003114	164	351	PS51207	PX-associated
	IPR000342	440	563	PS50132	Regulator of G protein signalling
	IPR001683	626	747	PS50195	Phox-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	32	EGAVAAVGGGPSSFRCCYGCCHE	54	SECONDARY	23
	2	66	VIMLTEASLSIWGWGSLGIVLFL	88	PRIMARY	23
	3	98	YLTFYILCFVGGGLVVTLLFGKT	120	PRIMARY	23