ORK00144

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK00144

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Accession No
Product ID	ORK00144
Clone name	ha04610
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of th ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
Symbol	NUP210
cDNA sequence	DNA sequence (6814 bp) Predicted protein sequence (1891 aa)
HaloTag ORF Clone	FHC00144
Flexi ORF Clone	FXC00144
Description	Nuclear pore membrane glycoprotein 210 precursor (POM210) (Nuclear pore protein gp210).

Features of the cloned cDNA sequence

Description

Length: 6814 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1044 bp
Genome contig ID	gi89161205r_13233137
PolyA signal sequence (None)	+--------+--------+----*----+---- AGGCCCTAGAGCCCGGCCTGGGCCAATGATGGAGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* GCGGCCGCCAGCCCAGGGCCTGCCCATCCAGAAGGGACTCCCCAGGGCCT

Features of the protein sequence

Description

Length: 1891 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG10397	100.0	nucleoporin 210...	synthetic construct
EAW64152	99.7	nucleoporin 210...	Homo sapiens
Q8TEM1	99.7	Nuclear pore me...	Homo sapiens
EAW64151	99.6	nucleoporin 210...	Homo sapiens
XP_001083137	97.2	similar to nucl...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003343	1081	1156	PF02368	Bacterial Ig-like
HMMSmart	IPR003343	1081	1156	SM00635	Bacterial Ig-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	10	RGLLLLTLSVLLAAGPSA	27	SECONDARY	18
	2	1485	ELSGAMVVGDVLCLATVLTSLEG	1507	PRIMARY	23
	3	1526	TGVAVARAVGSVTVYYEVAGHLR	1548	SECONDARY	23