ORK00171

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK00171

Order Kazusa clone(s) from :

Accession No
Product ID	ORK00171
Clone name	af06491
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	ABCA2
cDNA sequence	DNA sequence (8064 bp) Predicted protein sequence (2455 aa)
Flexi ORF Clone	FXC00171
Description	ATP-binding cassette sub-family A member 2 (ATP-binding cassette transporter 2) (ATP-binding cassette 2).

Features of the cloned cDNA sequence

Description

Length: 8064 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	695 bp
Genome contig ID	gi89161216r_138921507
PolyA signal sequence (AATAAA,-15)	+--------+--------+----*----+---- ACAGATGTTTGTTTTAAATAAATAAACAAAATGTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TGCGGGTCTGCGTGTGTGGGTTGGGAAGGGTGGGGAGGATGGGCGCATCA

Features of the protein sequence

Description

Length: 2455 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG10412	100.0	ATP-binding cas...	synthetic construct
CAI12768	99.8	ATP-binding cas...	Homo sapiens
NP_001597	99.8	ATP-binding cas...	Homo sapiens
AAG09372	99.8	ATP-binding cas...	Homo sapiens
Q9BZC7	99.7	ATP-binding cas...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	1142	1185	PD000006	ABC transporter related
BlastProDom	IPR003439	2205	2248	PD000006	ABC transporter related
HMMPfam	IPR003439	1037	1217	PF00005	ABC transporter related
HMMPfam	IPR003439	2100	2281	PF00005	ABC transporter related
HMMSmart	IPR003593	1036	1218	SM00382	AAA+ ATPase
HMMSmart	IPR003593	2099	2284	SM00382	AAA+ ATPase
ProfileScan	IPR003439	1010	1241	PS50893	ABC transporter related
ProfileScan	IPR003439	2070	2305	PS50893	ABC transporter related
ScanRegExp	IPR003439	1143	1157	PS00211	ABC transporter related
	IPR002345	1443	1456	PS00213	Lipocalin
	IPR013032	1660	1671	PS00022	EGF-like region

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	42	PWVLAFEIFIPLVLFFILLG	61	PRIMARY	20
	2	73	EAFYTAAPLTSAGILPVMQSLCP	95	SECONDARY	23
	3	721	FVIEHMMPLCMVISWVYSVAMTI	743	SECONDARY	23
	4	771	AWFITGFVQLSISVTALTAILKY	793	PRIMARY	23
	5	804	IIWLFLAVYAVATIMFCFLVSVL	826	PRIMARY	23
	6	832	LASACGGIIYFLSYVPYMYVAIR	854	PRIMARY	23
	7	867	KCIASLMSTTAFGLGSKYFALYE	889	SECONDARY	23
	8	910	FNLLLAVTMLMVDAVVYGILTWY	932	PRIMARY	23
	9	1807	LQGTDVVIAIFIIVAMSFVPASF	1829	PRIMARY	23
	10	1856	WLANYVWDMLNYLVPATCCVIIL	1878	PRIMARY	23
	11	1893	PAVLSLFLLYGWSITPIMYPASF	1915	PRIMARY	23
	12	1924	YVFLIVINLFIGITATVATFLLQ	1946	PRIMARY	23
	13	1954	LKVVNSYLKSCFLIFPNYNLGHG	1976	SECONDARY	23
	14	2007	IVTRGLVAMAVEGVVGFLLTIMC	2029	SECONDARY	23