ORK00323

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK00323

Order Kazusa clone(s) from :

Accession No	AB209989
Product ID	ORK00323
Clone name	ah00377
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	C4A
cDNA sequence	DNA sequence (5398 bp) Predicted protein sequence (1751 aa)
HaloTag ORF Clone	FHC00323
Flexi ORF Clone	FXC00323
Description	Complement component 4A (Rodgers blood group).

Features of the cloned cDNA sequence

Description

Length: 5398 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	123 bp
Genome contig ID	gi89161210f_32078419
PolyA signal sequence (ATTAAA,-19)	+--------+--------+----*----+---- CCTGGGACCAGGGCATATTAAAGGCTTTTGGCAGC
Flanking genome sequence (32739 - 32788)	----+--------+--------+--------+--------+----* AAAGTGTCAGTGTTGGCAGTGAAGTGTCAGTGTGTGTTGCTAGGGCTGAG

Features of the protein sequence

Description

Length: 1751 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAE06071	100.0	C4A variant pro...	Homo sapiens
CAI41782	100.0	complement comp...	Homo sapiens
CAQ09284	99.8	complement comp...	Homo sapiens
CAQ07657	99.8	complement comp...	Homo sapiens
P0C0L4	99.8	Complement C4-A...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000020	696	743	PD003264	Anaphylatoxin/fibulin
FPrintScan	IPR001840	705	714	PR00004	Complement C3a/C4a/C5a anaphylatoxin
	IPR001840	722	733	PR00004	Complement C3a/C4a/C5a anaphylatoxin
	IPR001840	734	745	PR00004	Complement C3a/C4a/C5a anaphylatoxin
HMMPfam	IPR002890	13	313	PF01835	Alpha-2-macroglobulin
	IPR011625	476	621	PF07703	Alpha-2-macroglobulin
	IPR000020	709	743	PF01821	Anaphylatoxin/fibulin
	IPR001599	788	880	PF00207	Alpha-2-macroglobulin
	IPR011626	1058	1324	PF07678	A-macroglobulin complement component
	IPR011627	1488	1577	PF07677	A-macroglobulin receptor
	IPR001134	1622	1732	PF01759	Netrin
HMMSmart	IPR000020	709	743	SM00104	Anaphylatoxin/fibulin
HMMSmart	IPR001134	1621	1733	SM00643	Netrin
ProfileScan	IPR000020	709	743	PS01178	Anaphylatoxin/fibulin
ProfileScan	IPR001134	1602	1749	PS50189	Netrin
ScanRegExp	IPR000020	709	743	PS01177	Anaphylatoxin/fibulin
ScanRegExp	IPR001599	1014	1022	PS00477	Alpha-2-macroglobulin

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	5	PPAMRLLWGLIWASSFFTLSLQ	26	SECONDARY	22
	2	31	LLFSPSVVHLGVPLSVGVQLQDV	53	SECONDARY	23
	3	878	PVEGLCLAGGGGLAQQVLVPAGS	900	SECONDARY	23
	4	902	RPVAFSVVPTAAAAVSLKVVARG	924	PRIMARY	23