ORK01009

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01009

Order Kazusa clone(s) from :

Accession No	AB210044
Product ID	ORK01009
Clone name	pg00769
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	ABCA3
cDNA sequence	DNA sequence (6450 bp) Predicted protein sequence (1708 aa)
HaloTag ORF Clone	FHC01009
Flexi ORF Clone	FXC01009
Description	ATP-binding cassette sub-family A member 3 (ATP-binding cassette transporter 3) (ATP-binding cassette 3) (ABC-C transporter).

Features of the cloned cDNA sequence

Description

Length: 6450 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	797 bp
Genome contig ID	gi51511732r_2165883
PolyA signal sequence (AATAAA,-25)	+--------+--------+----*----+---- CCATCCAGAAAATAAATGCTGAGGGGACACAAAAA
Flanking genome sequence (99996 - 99947)	----+--------+--------+--------+--------+----* CAGTCTGTGGGTCACACGCGCGAGCGGTCACAGGGCTCAATCACTCGCCC

Features of the protein sequence

Description

Length: 1708 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAE06126	100.0	ABCA3 variant p...	Homo sapiens
BAG10247	100.0	ATP-binding cas...	synthetic construct
XP_001162571	99.5	ATP-binding cas...	Pan troglodytes
Q99758	96.5	ATP-binding cas...	Homo sapiens
AAC50967	96.4	ABC3 [Homo sapi...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	669	711	PD000006	ABC transporter related
BlastProDom	IPR003439	1518	1561	PD000006	ABC transporter related
HMMPfam	IPR003439	563	743	PF00005	ABC transporter related
HMMPfam	IPR003439	1413	1594	PF00005	ABC transporter related
HMMSmart	IPR003593	562	744	SM00382	AAA+ ATPase
HMMSmart	IPR003593	1412	1596	SM00382	AAA+ ATPase
ProfileScan	IPR003439	534	767	PS50893	ABC transporter related
ProfileScan	IPR003439	1385	1618	PS50893	ABC transporter related
ScanRegExp	IPR003439	669	683	PS00211	ABC transporter related

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	19	AHLALFTLSFQSTFLLFPAGCSA	41	SECONDARY	23
	2	84	VLVTVLELFLPLLFSGILIWLR	105	PRIMARY	22
	3	325	LPLLLLLSFTYTALTIARAVVQE	347	SECONDARY	23
	4	369	AWFLLFFLFLLIAASFMTLLFCV	391	PRIMARY	23
	5	406	SLVLAFLLCFAISTISFSFMVST	428	PRIMARY	23
	6	451	FCFGQVLGMLLLDSVLYGLVTWY	473	PRIMARY	23
	7	931	AAQVLVPLTCVTLALLAINYSSE	953	SECONDARY	23
	8	1102	FDIALNLLFAMAFLASTFSILAV	1124	SECONDARY	23
	9	1145	FWLSALLWDLISFLIPSLLLLVV	1167	PRIMARY	23
	10	1186	LLLLLLYGWAIIPLMYLMNFFFL	1208	PRIMARY	23
	11	1218	TIFNILSGIATFLMVTIMRIPAV	1240	PRIMARY	23
	12	1308	GRFVASMAASGCAYLILLFLIET	1330	SECONDARY	23