ORK01019

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01019

Order Kazusa clone(s) from :

Accession No	AB210008
Product ID	ORK01019
Clone name	fg06458
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	ATP2B2
cDNA sequence	DNA sequence (6852 bp) Predicted protein sequence (1210 aa)
Flexi ORF Clone	FXC01019
Description	Plasma membrane calcium-transporting ATPase 2 (EC 3.6.3.8) (PMCA2) (Plasma membrane calcium pump isoform 2) (Plasma membrane calcium ATPase isoform 2).

Features of the cloned cDNA sequence

Description

Length: 6852 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	2743 bp
Genome contig ID	gi89161205r_10242755
PolyA signal sequence (AATAAA,-25)	+--------+--------+----*----+---- AATAATTGTAAATAAAGAACTCACCATCTCTGTTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTTAATACTATGCAATGGTTATGCTTTCAATCGCTGGCTCTTCTGACTC

Features of the protein sequence

Description

Length: 1210 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAE06090	100.0	ATP2B2 variant ...	Homo sapiens
EAW64079	100.0	ATPase, Ca++ tr...	Homo sapiens
AAA51893	99.8	plasma membrane...	Homo sapiens
AAA36456	99.5	Ca2+-ATPase [Ho...	Homo sapiens
XP_861393	99.2	similar to plas...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	249	263	PR00119	ATPase
	IPR006069	457	478	PR00121	ATPase
	IPR001757	464	478	PR00119	ATPase
	IPR006069	589	607	PR00121	ATPase
	IPR001757	668	679	PR00119	ATPase
	IPR001757	690	700	PR00119	ATPase
	IPR001757	785	804	PR00119	ATPase
	IPR001757	809	821	PR00119	ATPase
HMMPfam	IPR004014	53	136	PF00690	ATPase
	IPR008250	168	456	PF00122	E1-E2 ATPase-associated region
	IPR005834	460	808	PF00702	Haloacid dehalogenase-like hydrolase
	IPR006068	904	1055	PF00689	ATPase
HMMTigr	IPR006408	24	1054	TIGR01517	Calcium-translocating P-type ATPase
	IPR001757	168	290	TIGR01494	ATPase
	IPR001757	400	484	TIGR01494	ATPase
	IPR001757	665	715	TIGR01494	ATPase
	IPR001757	752	867	TIGR01494	ATPase
ScanRegExp	IPR001757	466	472	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	110	LVWEALQDVTLIILEIAAIISLG	132	SECONDARY	23
	2	161	AGWIEGAAILLSVICVVLVTAF	182	PRIMARY	22
	3	206	VVRAGQVVQIPVAEIVVGDIAQV	228	SECONDARY	23
	4	368	GLVMSAITVIILVLYFTVDTFVV	390	SECONDARY	23
	5	412	IIGVTVLVVAVPEGLPLAVTISL	434	PRIMARY	23
	6	847	QFQLTVNVVAVIVAFTGACITQD	869	PRIMARY	23
	7	924	GHAVYQLALIFTLLFVGEKMFQI	946	PRIMARY	23
	8	997	PIFCTIVLGTFAIQIVIVQFGGK	1019	PRIMARY	23
	9	1029	DQWMWCIFIGLGELVWGQVIATI	1051	PRIMARY	23