ORK01266

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01266

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01266
Clone name	ah01527
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRIK4
cDNA sequence	DNA sequence (5716 bp) Predicted protein sequence (957 aa)
HaloTag ORF Clone	FHC01266
Flexi ORF Clone	FXC01266
Description	Glutamate receptor, ionotropic kainate 4 precursor (Glutamate receptor KA-1) (KA1) (Excitatory amino acid receptor 1) (EAA1).

Features of the cloned cDNA sequence

Description

Length: 5716 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2549 bp
Genome contig ID	gi51511727f_119787669
PolyA signal sequence (AATAAA,-29)	+--------+--------+----*----+---- ATTTACAATAAAACAGAAACAAATCCTGAAATGTT
Flanking genome sequence (577061 - 577110)	----+--------+--------+--------+--------+----* AGTGATGGATGACAGCTGTTTGGGTTTAGAAAGTATGGTTCAGTGTGTCT

Features of the protein sequence

Description

Length: 957 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW67512	100.0	glutamate recep...	Homo sapiens
BAG36943	99.8	unnamed protein...	Homo sapiens
Q16099	99.7	Glutamate recep...	Homo sapiens
Q5IS46	99.8	Glutamate recep...	Pan troglodytes
XP_001106948	99.7	similar to glut...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	615	681	PD245531	NULL
FPrintScan	IPR001508	462	490	PR00177	NMDA receptor
	IPR001508	548	573	PR00177	NMDA receptor
	IPR001508	621	648	PR00177	NMDA receptor
	IPR001508	806	830	PR00177	NMDA receptor
HMMPfam	IPR001828	41	384	PF01094	Extracellular ligand-binding receptor
HMMPfam	IPR001320	545	827	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	418	787	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	3	PRVSAPLVLLPAWLVMVACSPHS	25	PRIMARY	23
	2	548	VWLFMLLAYLAVSCVLFLVARLT	570	PRIMARY	23
	3	626	WWAFTLIIISSYTANLAAFLTVQ	648	PRIMARY	23
	4	805	NIGGIFVVLICGLIVAIFMAMLE	827	PRIMARY	23