ORK01285

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01285

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01285
Clone name	ha01215
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of th ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
Symbol	ATP2A3
cDNA sequence	DNA sequence (4041 bp) Predicted protein sequence (1043 aa)
HaloTag ORF Clone	FHC01285
Flexi ORF Clone	FXC01285
Description	ATPase, Ca++ transporting, ubiquitous

Features of the cloned cDNA sequence

Description

Length: 4041 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	908 bp
Genome contig ID	gi51511734r_3674557
PolyA signal sequence (None)	+--------+--------+----*----+---- TCCTGACCGTGGCGCCCTTCTACCCAGCCCAGTGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* GCGGCCGCCGCGCTGTCTGCACAGCTGGGGGCCTCTGAGCCTGGTGGGCT

Features of the protein sequence

Description

Length: 1043 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAH35729	100.0	ATPase, Ca++ tr...	Homo sapiens
CAA93737	99.9	adenosine triph...	Homo sapiens
EAW90468	99.9	ATPase, Ca++ tr...	Homo sapiens
AAC24525	99.8	sarco-/endoplas...	Homo sapiens
BAF85405	99.8	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	220	234	PR00119	ATPase
	IPR001757	393	407	PR00119	ATPase
	IPR001757	639	650	PR00119	ATPase
	IPR001757	661	671	PR00119	ATPase
	IPR000695	717	733	PR00120	H+ transporting ATPase
	IPR000695	745	761	PR00120	H+ transporting ATPase
	IPR001757	745	764	PR00119	ATPase
	IPR001757	768	780	PR00119	ATPase
	IPR000695	776	801	PR00120	H+ transporting ATPase
HMMPfam	IPR004014	38	121	PF00690	ATPase
	IPR008250	137	385	PF00122	E1-E2 ATPase-associated region
	IPR005834	389	768	PF00702	Haloacid dehalogenase-like hydrolase
	IPR006068	863	1034	PF00689	ATPase
HMMTigr	IPR005782	97	1033	TIGR01116	Calcium ATPase
	IPR001757	137	240	TIGR01494	ATPase
	IPR001757	289	420	TIGR01494	ATPase
	IPR001757	636	686	TIGR01494	ATPase
	IPR001757	713	837	TIGR01494	ATPase
ScanRegExp	IPR001757	395	401	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	102	EDLLVRILLLAALVSFVLAWFE	123	PRIMARY	22
	2	130	TAFVEPLVIMLILVANAIVGVWQ	152	PRIMARY	23
	3	302	RQLSHAISVICVAVWVINIGHFA	324	PRIMARY	23
	4	343	AVALAVAAIPEGLPAVITTCLAL	365	PRIMARY	23
	5	820	FLTAILGLPEALIPVQLLWVNLV	842	SECONDARY	23
	6	875	GWLFFRYLAIGVYVGLATVAAAT	897	PRIMARY	23
	7	939	FPTTMALSVLVTIEMCNALNSVS	961	SECONDARY	23
	8	977	LLVAVAMSMALHFLILLVPPLPL	999	PRIMARY	23
	9	1010	QWVVVLQISLPVILLDEALKYLS	1032	PRIMARY	23