ORK01303

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01303

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01303
Clone name	hk10385
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	ATP1A1
cDNA sequence	DNA sequence (3650 bp) Predicted protein sequence (1038 aa)
HaloTag ORF Clone	FHC01303
Flexi ORF Clone	FXC01303
Description	Sodium/potassium-transporting ATPase subunit alpha-1 precursor (EC 3.6.3.9) (Sodium pump subunit alpha-1) (Na(+)/K(+) ATPase alpha-1 subunit).

Features of the cloned cDNA sequence

Description

Length: 3650 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	328 bp
Genome contig ID	gi89161185f_116617407
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- TGGATTTTTACAAATAAAGATGGCTATTATAATGG
Flanking genome sequence (131512 - 131561)	----+--------+--------+--------+--------+----* AATTTGTCTGTGCCCTCGTCGCTTCTTTGGTACAGATGTCTATGTGATCT

Features of the protein sequence

Description

Length: 1038 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
P05023	100.0	Sodium/potassiu...	Homo sapiens
BAG37313	99.9	unnamed protein...	Homo sapiens
ABW03450	99.9	ATPase, Na+/K+ ...	synthetic construct
XP_001112674	99.7	Na+/K+ -ATPase ...	Macaca mulatta
XP_513679	99.9	hypothetical pr...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR006069	118	132	PR00121	ATPase
	IPR006069	145	165	PR00121	ATPase
	IPR001757	229	243	PR00119	ATPase
	IPR006069	309	331	PR00121	ATPase
	IPR006069	382	403	PR00121	ATPase
	IPR001757	389	403	PR00119	ATPase
	IPR006069	520	538	PR00121	ATPase
	IPR001757	602	613	PR00119	ATPase
	IPR001757	624	634	PR00119	ATPase
	IPR001757	730	749	PR00119	ATPase
	IPR001757	754	766	PR00119	ATPase
	IPR006069	802	823	PR00121	ATPase
	IPR006069	869	889	PR00121	ATPase
	IPR006069	931	951	PR00121	ATPase
	IPR006069	965	989	PR00121	ATPase
HMMPfam	IPR004014	48	131	PF00690	ATPase
	IPR008250	150	381	PF00122	E1-E2 ATPase-associated region
	IPR005834	385	753	PF00702	Haloacid dehalogenase-like hydrolase
	IPR006068	849	1027	PF00689	ATPase
HMMTigr	IPR005775	42	1038	TIGR01106	ATPase
	IPR001757	150	242	TIGR01494	ATPase
	IPR001757	294	420	TIGR01494	ATPase
	IPR001757	599	649	TIGR01494	ATPase
	IPR001757	704	823	TIGR01494	ATPase
ScanRegExp	IPR001757	391	397	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	113	GGFSMLLWIGAILCFLAYSIQAA	135	PRIMARY	23
	2	145	LYLGVVLSAVVIITGCFSYYQ	165	PRIMARY	21
	3	307	IHIITGVAVFLGVSFFILSLILE	329	PRIMARY	23
	4	339	LIGIIVANVPEGLLATVTVCLTL	361	PRIMARY	23
	5	810	IANIPLPLGTVTILCIDLGTDMV	832	SECONDARY	23
	6	874	MIQALGGFFTYFVILAENGFLPI	896	SECONDARY	23
	7	930	EFTCHTAFFVSIVVVQWADLVIC	952	PRIMARY	23
	8	968	ILIFGLFEETALAAFLSYCPGMG	990	SECONDARY	23
	9	998	LKPTWWFCAFPYSLLIFVYDEV	1019	SECONDARY	22