ORK01333

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01333

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01333
Clone name	hh15786
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GABRB2
cDNA sequence	DNA sequence (6161 bp) Predicted protein sequence (495 aa)
HaloTag ORF Clone	FHC01333
Flexi ORF Clone	FXC01333
Description	Gamma-aminobutyric-acid receptor subunit beta-2 precursor (GABA(A) receptor subunit beta-2).

Features of the cloned cDNA sequence

Description

Length: 6161 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	4671 bp
Genome contig ID	gi51511721r_160548994
PolyA signal sequence (None)	+--------+--------+----*----+---- CCTTTCAACCAAAGACTCCAGAATTATTGAAAAAC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAAGGAAAAAAAAAATGAGATAATGGGGGAAAAAAAGGACGCTGCTT

Features of the protein sequence

Description

Length: 495 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
P47870	2.8e-206	100.0	Gamma-aminobuty...	Homo sapiens
P63138	5.2e-206	99.7	Gamma-aminobuty...	Rattus norvegicus
XP_867070	8.4e-206	99.5	similar to Gamm...	Canis lupus fam...
BAF82419	1.1e-205	99.7	unnamed protein...	Homo sapiens
XP_001143814	2.5e-205	99.5	gamma-aminobuty...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR006201	103	119	PR00252	Neurotransmitter-gated ion-channel
	IPR002289	116	130	PR01160	Gamma-aminobutyric-acid A receptor
	IPR006201	135	146	PR00252	Neurotransmitter-gated ion-channel
	IPR006201	181	195	PR00252	Neurotransmitter-gated ion-channel
	IPR002289	220	233	PR01160	Gamma-aminobutyric-acid A receptor
	IPR006201	257	269	PR00252	Neurotransmitter-gated ion-channel
	IPR006028	266	286	PR00253	Gamma-aminobutyric acid A receptor
	IPR006028	292	313	PR00253	Gamma-aminobutyric acid A receptor
	IPR002289	320	332	PR01160	Gamma-aminobutyric-acid A receptor
	IPR006028	326	347	PR00253	Gamma-aminobutyric acid A receptor
	IPR002289	461	475	PR01160	Gamma-aminobutyric-acid A receptor
	IPR006028	473	493	PR00253	Gamma-aminobutyric acid A receptor
HMMPfam	IPR006202	57	263	PF02931	Neurotransmitter-gated ion-channel ligand-binding
HMMPfam	IPR006029	270	490	PF02932	Neurotransmitter-gated ion-channel transmembrane region
HMMTigr	IPR006201	22	493	TIGR00860	Neurotransmitter-gated ion-channel
ScanRegExp	IPR006201	181	195	PS00236	Neurotransmitter-gated ion-channel

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	29	GYFGIWSFPLIIAAVCAQSVNDP	51	SECONDARY	23
	2	264	GYFILQTYMPSILITILSWVSFW	286	PRIMARY	23
	3	329	YLMGCFVFVFMALLEYALVNYIF	351	PRIMARY	23
	4	473	WSRIFFPVVFSFFNIVYWLYYVN	495	PRIMARY	23