ORK01340

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01340

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01340
Clone name	aj00720
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	SLC4A2
cDNA sequence	DNA sequence (4925 bp) Predicted protein sequence (1244 aa)
HaloTag ORF Clone	FHC01340
Flexi ORF Clone	FXC01340
Description	solute carrier family 4, anion exchanger, member 2 (erythrocyte membrane protein band 3-like 1)

Features of the cloned cDNA sequence

Description

Length: 4925 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	159 bp
Genome contig ID	gi89161213f_150286232
PolyA signal sequence (AGTAAA,-24)	+--------+--------+----*----+---- ACTGCCCCTGCAGTAAAGTGCTTTGGCCCCCACCT
Flanking genome sequence (118316 - 118365)	----+--------+--------+--------+--------+----* ATCTGTGGCCTTTTGTCTTTGTATGGGGGGCGGGGCATGAATGGAAGAGG

Features of the protein sequence

Description

Length: 1244 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92395	100.0	Anion exchanger...	Homo sapiens
P04920	100.0	Anion exchange ...	Homo sapiens
AAF19583	99.8	anion exchanger...	Homo sapiens
AAC50964	99.7	AE2 anion excha...	Homo sapiens
Q5RD44	99.5	Anion exchange ...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR002978	20	36	PR01188	Anion exchanger
	IPR002978	51	64	PR01188	Anion exchanger
	IPR002978	304	320	PR01188	Anion exchanger
	IPR001717	422	427	PR00165	Anion exchange protein
	IPR002978	463	482	PR01188	Anion exchanger
	IPR003020	553	565	PR01231	HCO3- transporter
	IPR003020	701	710	PR01231	HCO3- transporter
	IPR001717	710	717	PR00165	Anion exchange protein
	IPR001717	720	726	PR00165	Anion exchange protein
	IPR003020	726	737	PR01231	HCO3- transporter
	IPR001717	743	750	PR00165	Anion exchange protein
	IPR001717	783	791	PR00165	Anion exchange protein
	IPR003020	816	828	PR01231	HCO3- transporter
	IPR003020	837	849	PR01231	HCO3- transporter
	IPR001717	839	846	PR00165	Anion exchange protein
	IPR001717	902	912	PR00165	Anion exchange protein
	IPR001717	915	924	PR00165	Anion exchange protein
	IPR003020	940	949	PR01231	HCO3- transporter
	IPR001717	985	993	PR00165	Anion exchange protein
	IPR003020	1009	1018	PR01231	HCO3- transporter
	IPR003020	1023	1036	PR01231	HCO3- transporter
	IPR001717	1039	1047	PR00165	Anion exchange protein
	IPR001717	1068	1076	PR00165	Anion exchange protein
HMMPfam	IPR013769	355	623	PF07565	HCO3- transporter
HMMPfam	IPR011531	677	1172	PF00955	HCO3-transporter
HMMTigr	IPR003020	327	1243	TIGR00834	HCO3- transporter
ScanRegExp	IPR001717	685	696	PS00219	Anion exchange protein
ScanRegExp	IPR001717	832	846	PS00220	Anion exchange protein

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	709	PQCLAAVIFIYFAALSPAITFGG	731	PRIMARY	23
	2	739	DLIGVSELIMSTALQGVVFCLLG	761	SECONDARY	23
	3	765	LLVIGFSGPLLVFEEAFFSFCSS	787	SECONDARY	23
	4	797	VWIGFWLVFLALLMVALEGSFLV	819	PRIMARY	23
	5	828	EIFAFLISLIFIYETFYKLVKIF	850	PRIMARY	23
	6	903	TALLSLVLMAGTFFIAFFL	921	PRIMARY	19
	7	937	VIGDFGVPIAILIMVLVDYSIED	959	PRIMARY	23
	8	996	MMVASLLPAILVFILIFMETQIT	1018	PRIMARY	23
	9	1038	DLLLIVAMGGICALFGLPWLAAA	1060	PRIMARY	23
	10	1104	GLSIVIGDLLRQIPLAVLFGIFL	1126	PRIMARY	23
	11	1163	TLRMHLFTALQLLCLALLWAVMS	1185	PRIMARY	23
	12	1191	AFPFILILTVPLRMVVLTRIFTD	1213	PRIMARY	23