ORK01364

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01364

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01364
Clone name	fk01449
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	TRPC4
cDNA sequence	DNA sequence (3190 bp) Predicted protein sequence (978 aa)
HaloTag ORF Clone	FHC01364
Flexi ORF Clone	FXC01364
Description	Short transient receptor potential channel 4 (TrpC4) (Trp-related protein 4) (hTrp-4) (hTrp4).

Features of the cloned cDNA sequence

Description

Length: 3190 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	93 bp
Genome contig ID	gi51511729r_37008947
PolyA signal sequence (None)	+--------+--------+----*----+---- GGGGAAAATGTTTAAATTGTATTAGCAAATGCTAA
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* CTTACACTTTATAGCGTTTATCAGCTGTGGCATATTACCTGTAACATGTT

Features of the protein sequence

Description

Length: 978 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9UBN4	100.0	Short transient...	Homo sapiens
AAI05642	99.8	Transient recep...	Homo sapiens
BAG35423	99.8	unnamed protein...	Homo sapiens
AAL24551	99.4	transient recep...	Homo sapiens
XP_543129	98.1	similar to Shor...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR002110	143	155	PR01415	Ankyrin
	IPR002110	155	167	PR01415	Ankyrin
	IPR002153	603	624	PR01097	Transient receptor potential protein
	IPR002153	625	638	PR01097	Transient receptor potential protein
	IPR002153	653	666	PR01097	Transient receptor potential protein
	IPR005460	756	767	PR01645	Transient receptor potential channel 4
	IPR005460	775	791	PR01645	Transient receptor potential channel 4
	IPR005460	793	815	PR01645	Transient receptor potential channel 4
	IPR005460	818	840	PR01645	Transient receptor potential channel 4
	IPR005460	842	855	PR01645	Transient receptor potential channel 4
	IPR005460	899	928	PR01645	Transient receptor potential channel 4
HMMPfam	IPR002110	70	92	PF00023	Ankyrin
	IPR002110	142	169	PF00023	Ankyrin
	IPR013555	177	239	PF08344	Transient receptor ion channel II
	IPR005821	405	621	PF00520	Ion transport
HMMSmart	IPR002110	70	99	SM00248	Ankyrin
HMMSmart	IPR002110	142	171	SM00248	Ankyrin
HMMTigr	IPR004729	18	750	TIGR00870	Transient receptor potential channel
ProfileScan	IPR002110	142	174	PS50088	Ankyrin
ProfileScan	IPR002110	142	174	PS50297	Ankyrin
ScanRegExp	IPR001865	493	504	PS00962	Ribosomal protein S2

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	329	VKMVTCFIIGLLFPVFSVCYLIA	351	PRIMARY	23
	2	366	KFICHTASYLTFLFLLLLASQHI	388	SECONDARY	23
	3	399	PPTIVEWMILPWVLGFIWGEIKQ	421	SECONDARY	23
	4	438	MDFVMNSLYLATISLKIVAFVKY	460	SECONDARY	23
	5	475	TLVAEALFAIANIFSSLRLISLF	497	SECONDARY	23
	6	512	RMLLDILKFLFIYCLVLLAFANG	534	PRIMARY	23
	7	569	LQSLFWSIFGLINLYVTNVKAQH	591	SECONDARY	23
	8	597	VGATMFGTYNVISLVVLLNMLIA	619	SECONDARY	23