ORK01427

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01427

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Accession No	AB209567
Product ID	ORK01427
Clone name	fk14761
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRIA2
cDNA sequence	DNA sequence (3459 bp) Predicted protein sequence (911 aa)
HaloTag ORF Clone	FHC01427
Flexi ORF Clone	FXC01427
Description	Glutamate receptor 2 precursor (GluR-2) (GluR-B) (GluR-K2) (Glutamate receptor ionotropic, AMPA 2) (AMPA-selective glutamate receptor 2).

Features of the cloned cDNA sequence

Description

Length: 3459 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	519 bp
Genome contig ID	gi89161207f_158261356
PolyA signal sequence (None)	+--------+--------+----*----+---- TGACATCAACTGCATCAAGATGTGACATGTTTTAT
Flanking genome sequence (243198 - 243247)	----+--------+--------+--------+--------+----* AAAAAAAGGAAAAAAAACATTTAAAACTAAAAAATATTTTTAGGTATTTT

Features of the protein sequence

Description

Length: 911 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92804	100.0	glutamate recep...	Homo sapiens
BAG10835	100.0	glutamate recep...	synthetic construct
EAX04865	99.8	glutamate recep...	Homo sapiens
BAE06155	99.7	AMPA-selective ...	Mus musculus
AAA58631	99.7	glutamate recep...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	643	709	PD245531	NULL
FPrintScan	IPR001508	487	515	PR00177	NMDA receptor
	IPR001508	649	676	PR00177	NMDA receptor
	IPR001508	841	865	PR00177	NMDA receptor
HMMPfam	IPR001828	84	408	PF01094	Extracellular ligand-binding receptor
HMMPfam	IPR001320	571	862	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	443	818	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	19	YIVDALLFLEMQKIMHISVLLSP	41	SECONDARY	23
	2	568	DPLAYEIWMCIVFAYIGVSVVLF	590	PRIMARY	23
	3	654	WWFFTLIIISSYTANLAAFLTVE	676	PRIMARY	23
	4	839	SNVAGVFYILVGGLGLAMLVALI	861	PRIMARY	23