ORK01572

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01572

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01572
Clone name	hk05555
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	NNT
cDNA sequence	DNA sequence (4495 bp) Predicted protein sequence (1135 aa)
HaloTag ORF Clone	FHC01572
Flexi ORF Clone	FXC01572
Description	NAD(P) transhydrogenase, mitochondrial precursor (EC 1.6.1.2) (Pyridine nucleotide transhydrogenase) (Nicotinamide nucleotide transhydrogenase).

Features of the cloned cDNA sequence

Description

Length: 4495 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	1087 bp
Genome contig ID	gi51511721f_43539060
PolyA signal sequence (AGTAAA,-24)	+--------+--------+----*----+---- ATTTAAGCACCAGTAAAGATCATGTCTTTTTATAG
Flanking genome sequence (202292 - 202341)	----+--------+--------+--------+--------+----* AAGTGTAGATTTTCTTTGTGACTTTGCTATCGTGCCTAAAGCTCTAAATA

Features of the protein sequence

Description

Length: 1135 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q13423	100.0	NAD(P) transhyd...	Homo sapiens
AAI10545	99.9	Nicotinamide nu...	Homo sapiens
XP_001134902	99.8	nicotinamide nu...	Pan troglodytes
CAD38536	99.8	hypothetical pr...	Homo sapiens
CAL37542	99.7	hypothetical pr...	synthetic construct

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR007886	109	248	PF05222	Alanine dehydrogenase/PNT
	IPR007698	257	427	PF01262	Alanine dehydrogenase/PNT
	IPR004003	667	1129	PF02233	NAD(P) transhydrogenase
HMMTigr	IPR004571	107	637	TIGR00561	NAD(P) transhydrogenase
ScanRegExp	IPR008142	109	135	PS00836	Alanine dehydrogenase/PNT
ScanRegExp	IPR008143	281	306	PS00837	Alanine dehydrogenase/PNT

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	528	YTAGLTGILGLGIAAPNLAFSQM	550	SECONDARY	23
	2	554	FGLAGIVGYHTVWGVTPALHSPL	576	SECONDARY	23
	3	580	TNAISGLTAVGGLALMGGHLYPS	602	SECONDARY	23
	4	646	LYLLPAGTFVGGYLAALYSGYNI	668	SECONDARY	23
	5	670	QIMYLGSGLCCVGALAGLSTQGT	692	SECONDARY	23
	6	695	LGNALGMIGVAGGLAATLGVLKP	717	SECONDARY	23
	7	719	PELLAQMSGAMALGGTIGLTIA	740	SECONDARY	22
	8	750	QLVAAFHSLVGLAAVLTCIAEYI	772	SECONDARY	23
	9	785	NLTKIVAYLGTYIGGVTFSGSLI	807	SECONDARY	23
	10	821	LLLPGRHLLNAGLLAASVGGIIP	843	SECONDARY	23
	11	860	VSALSAVMGVTLTAAIGGADMPV	882	SECONDARY	23
	12	907	LTIVGALIGSSGAILSYIMCVAM	929	PRIMARY	23
	13	973	ANSIIITPGYGLCAAKAQYPIAD	995	SECONDARY	23