ORK01710

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01710

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Accession No
Product ID	ORK01710
Clone name	ha02933
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of th ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
Symbol	FZD1
cDNA sequence	DNA sequence (4670 bp) Predicted protein sequence (762 aa)
HaloTag ORF Clone	FHC01710
Flexi ORF Clone	FXC01710
Description	Frizzled-1 precursor (Fz-1) (hFz1) (FzE1).

Features of the cloned cDNA sequence

Description

Length: 4670 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2380 bp
Genome contig ID	gi89161213f_90631787
PolyA signal sequence (CATAAA,-19)	+--------+--------+----*----+---- TAACTGGCTATGTGTTCATAAACATTTTAAACAGC
Flanking genome sequence (104670 - 104719)	----+--------+--------+--------+--------+----* AATTGGGTTTGTAAGGCTGTTTTGTTGTTTGCTTCTGAAGACCCCTTTTT

Features of the protein sequence

Description

Length: 762 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
AAD41636	0	100.0	frizzled 1 [Hom...	Homo sapiens
Q9UP38	0	99.8	Frizzled-1; Sho...	Homo sapiens
BAG37492	0	99.6	unnamed protein...	Homo sapiens
ABY63622	0	98.9	frizzled homolo...	Papio anubis
XP_519190	1e-216	99.0	frizzled 1 [Pan...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000539	437	459	PR00489	Frizzled protein
	IPR000539	465	487	PR00489	Frizzled protein
	IPR000539	519	543	PR00489	Frizzled protein
	IPR000539	562	585	PR00489	Frizzled protein
	IPR000539	607	629	PR00489	Frizzled protein
	IPR000539	648	669	PR00489	Frizzled protein
	IPR000539	717	737	PR00489	Frizzled protein
HMMPfam	IPR000024	225	343	PF01392	Frizzled CRD region
HMMPfam	IPR000539	424	755	PF01534	Frizzled protein
HMMSmart	IPR000024	230	347	SM00063	Frizzled CRD region
ProfileScan	IPR000024	226	345	PS50038	Frizzled CRD region
ProfileScan	IPR000832	432	744	PS50261	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	436	TWIGIWSVLCCASTLFTVLTYLV	458	PRIMARY	23
	2	465	YPERPIIFLSGCYTAVAVAYIAG	487	SECONDARY	23
	3	521	FMMLYFFSMASSIWWVILSLTWF	543	PRIMARY	23
	4	562	FHLAAWAVPAIKTITILALGQVD	584	SECONDARY	23
	5	607	LAPLFVYLFIGTSFLLAGFVSLF	629	PRIMARY	23
	6	649	MVRIGVFSVLYTVPATIVIACYF	671	PRIMARY	23