ORK01816

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01816

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01816
Clone name	bm05381
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	PPAPDC3
cDNA sequence	DNA sequence (2068 bp) Predicted protein sequence (312 aa)
HaloTag ORF Clone	FHC01816
Flexi ORF Clone	FXC01816
Description	Probable lipid phosphate phosphatase PPAPDC3 (EC 3.1.3.-) (Phosphatidic acid phosphatase type 2 domain-containing protein 3).

Features of the cloned cDNA sequence

Description

Length: 2068 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	974 bp
Genome contig ID	gi89161216f_133054928
PolyA signal sequence (AATACA,-23)	+--------+--------+----*----+---- ATTTCATGTGACAATACAGATGACATGCAAATGGC
Flanking genome sequence (119543 - 119592)	----+--------+--------+--------+--------+----* CCTCTCCCGTGTGGCTTTTTCCTCATGCAATTCAATTGCTCTGGATGCAT

Features of the protein sequence

Description

Length: 312 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
AAH06362	2.2e-98	100.0	Phosphatidic ac...	Homo sapiens
EAW87966	2.5e-98	100.0	phosphatidic ac...	Homo sapiens
Q8NBV4	6.4e-98	99.6	Probable lipid ...	Homo sapiens
BAE88177	7.3e-98	99.2	unnamed protein...	Macaca fascicularis
BAB55204	1.1e-97	99.2	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000326	185	304	PF01569	Phosphoesterase
HMMSmart	IPR000326	184	296	SM00014	Phosphoesterase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	29	RAGIGLLAVSEVTMPA	44	SECONDARY	16
	2	155	KLIGITGHGIPWIGGTILCLVKS	177	SECONDARY	23
	3	184	EVLMNLLLALLLDIMTVAGVQKL	206	PRIMARY	23
	4	245	FLSHLVLAVPLRVLLVLWALCVG	267	PRIMARY	23
	5	284	GFVIGYLQFRLVELVWMPSSTCQ	306	SECONDARY	23