ORK01878

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01878

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01878
Clone name	ef01296
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	SLC12A6
cDNA sequence	DNA sequence (7700 bp) Predicted protein sequence (1186 aa)
HaloTag ORF Clone	FHC01878
Flexi ORF Clone	FXC01878
Description	Solute carrier family 12 (potassium/chloride transporters), member 6

Features of the cloned cDNA sequence

Description

Length: 7700 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	2984 bp
Genome contig ID	gi51511731r_32209490
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- CTGGAAACAAAAATAAAGTTTTCTACATTATTTTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGCCTTGGGTTATGGTATAGTTTCTTTGTGTTTGTCGTAATATGCACATT

Features of the protein sequence

Description

Length: 1186 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9UHW9	100.0	Solute carrier ...	Homo sapiens
XP_001173480	99.9	solute carrier ...	Pan troglodytes
AAF24986	99.9	K-Cl cotranspor...	Homo sapiens
BAF85239	99.7	unnamed protein...	Homo sapiens
ABA02873	100.0	potassium chlor...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000076	167	175	PR01081	K-Cl co-transporter
	IPR000076	368	380	PR01081	K-Cl co-transporter
	IPR000076	403	420	PR01081	K-Cl co-transporter
	IPR000076	641	657	PR01081	K-Cl co-transporter
	IPR000076	667	675	PR01081	K-Cl co-transporter
HMMPfam	IPR004841	226	797	PF00324	Amino acid permease-associated region
HMMPfam	IPR000622	1054	1083	PF03522	K-Cl Co-transporter
HMMTigr	IPR004842	145	1186	TIGR00930	Na-K-Cl cotransporter superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	224	FMGVYLPCLQNIFGVILFLRLTW	246	PRIMARY	23
	2	259	IVLICCCCTMLTAISMSAIATNG	281	PRIMARY	23
	3	309	YLGTTFAAAMYILGAIEIFLVYI	331	SECONDARY	23
	4	356	VYGTAFLVLMVLVVFIGVRYVNK	378	PRIMARY	23
	5	385	ACVIVSILAIYAGAIKSSFAPPH	407	PRIMARY	23
	6	515	VDITTSFTLLVGIFFPSVTGIMA	537	SECONDARY	23
	7	557	ILAILTTSFVYLSNVVLFGACIE	579	PRIMARY	23
	8	594	NLVVGTLSWPSPWVIVIGSFFST	616	SECONDARY	23
	9	657	WALLLTAAIAELGILIASLDLVA	679	PRIMARY	23
	10	684	MFFLMCYLFVNLACALQTLLRTP	706	PRIMARY	23
	11	723	GMSICLALMFISSWYYAIVAMVI	745	PRIMARY	23