ORK01913

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01913

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Accession No
Product ID	ORK01913
Clone name	ha00575
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of th ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
Symbol	ATP13A2
cDNA sequence	DNA sequence (3978 bp) Predicted protein sequence (1180 aa)
HaloTag ORF Clone	FHC01913
Flexi ORF Clone	FXC01913
Description	Probable cation-transporting ATPase 13A2 (EC 3.6.3.-).

Features of the cloned cDNA sequence

Description

Length: 3978 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	263 bp
Genome contig ID	gi89161185r_17085040
PolyA signal sequence (AATAAA,-25)	+--------+--------+----*----+---- CAGTGTAGCAAATAAAGTCATGATATTTTCCTGGC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TCTGCTGTGCCTGTCTTGTACCTGGCACCATAGACCCTCCCACCTGCCCA

Features of the protein sequence

Description

Length: 1180 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAR23423	100.0	putative N-ATPa...	Homo sapiens
Q9NQ11	99.5	Probable cation...	Homo sapiens
XP_001087655	97.1	similar to ATPa...	Macaca mulatta
AAH34575	99.5	ATP13A2 protein...	Homo sapiens
EAW94824	100.0	ATPase type 13A...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	341	355	PR00119	ATPase
	IPR001757	511	525	PR00119	ATPase
	IPR001757	876	895	PR00119	ATPase
HMMPfam	IPR008250	299	387	PF00122	E1-E2 ATPase-associated region
HMMPfam	IPR005834	507	765	PF00702	Haloacid dehalogenase-like hydrolase
HMMTigr	IPR006544	37	1123	TIGR01657	P-type ATPase of unknown pump specificity (type V)
	IPR001757	262	532	TIGR01494	ATPase
	IPR001757	850	966	TIGR01494	ATPase
ScanRegExp	IPR001757	513	519	PS00154	ATPase
ScanRegExp	IPR000150	876	898	PS01229	Cof protein

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	249	ALWLADHYYWYALCIFLISSISI	271	PRIMARY	23
	2	427	KFVAALSVLALLGTIYSIFILY	448	PRIMARY	22
	3	460	RALDLVTVVVPPALPAAMTVCTL	482	SECONDARY	23
	4	933	VFKYMALYSLTQFISVLILYTIN	955	PRIMARY	23
	5	993	PGALLSVPVLSSLLLQMVLVTGV	1015	SECONDARY	23
	6	1047	NTVVFSLSSFQYLILAAAVSKGA	1069	SECONDARY	23
	7	1080	PFLVALALLSSVLVGLVLVPGLL	1102	PRIMARY	23
	8	1119	LLLGLVTLNFVGAFMLESVLDQC	1141	SECONDARY	23