ORK01936

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK01936

Order Kazusa clone(s) from :

Accession No
Product ID	ORK01936
Clone name	ph01207
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	ADGRB2
cDNA sequence	DNA sequence (5119 bp) Predicted protein sequence (1547 aa)
HaloTag ORF Clone	FHC01936
Flexi ORF Clone	FXC01936
Description	Brain-specific angiogenesis inhibitor 2 precursor.

Features of the cloned cDNA sequence

Description

Length: 5119 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	302 bp
Genome contig ID	gi89161185r_31865353
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- GGGGAACTTTGTTGGGAATAAACTTCACTCTGTGG
Flanking genome sequence (99953 - 99904)	----+--------+--------+--------+--------+----* ATTTTGCTGCTGAGCTGTTTGCTTCCCTCAGGTAAAGGCCCAGCTCTGGG

Features of the protein sequence

Description

Length: 1547 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG10259	100.0	brain-specific ...	synthetic construct
CAM12876	98.7	brain-specific ...	Homo sapiens
XP_001160345	98.0	brain-specific ...	Pan troglodytes
CAQ52064	95.4	brain-specific ...	Mus musculus
CAQ51597	95.3	brain-specific ...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR008077	82	99	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	519	531	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	539	555	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	596	615	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	638	647	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	699	720	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	896	920	PR00249	GPCR
	IPR008077	920	930	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	960	983	PR00249	GPCR
	IPR008077	984	997	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	997	1022	PR00249	GPCR
	IPR008077	1023	1040	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	1040	1065	PR00249	GPCR
	IPR000832	1115	1135	PR00249	GPCR
	IPR008077	1136	1146	PR01694	Brain-specific angiogenesis inhibitor
	IPR000832	1146	1167	PR00249	GPCR
	IPR008077	1200	1213	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	1420	1434	PR01694	Brain-specific angiogenesis inhibitor
	IPR008077	1437	1451	PR01694	Brain-specific angiogenesis inhibitor
HMMPfam	IPR000884	330	378	PF00090	Thrombospondin
	IPR000884	385	433	PF00090	Thrombospondin
	IPR000884	441	489	PF00090	Thrombospondin
	IPR001879	493	554	PF02793	Hormone receptor
	IPR000203	832	880	PF01825	GPS
	IPR000832	891	1171	PF00002	GPCR
HMMSmart	IPR000884	329	379	SM00209	Thrombospondin
	IPR000884	384	434	SM00209	Thrombospondin
	IPR000884	440	490	SM00209	Thrombospondin
	IPR001879	492	558	SM00008	Hormone receptor
	IPR000203	832	885	SM00303	GPS
ProfileScan	IPR000884	326	379	PS50092	Thrombospondin
	IPR000884	381	434	PS50092	Thrombospondin
	IPR000884	437	490	PS50092	Thrombospondin
	IPR001879	483	553	PS50227	Hormone receptor
	IPR000203	833	885	PS50221	GPS
	IPR000832	894	1168	PS50261	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	29	RMTPACPLLLSVILSLRLATAFD	51	SECONDARY	23
	2	774	SYFVIGAVLYRTLGLILPPP	793	SECONDARY	20
	3	897	PLVIGCAVSCMALLTLLAIYAAF	919	PRIMARY	23
	4	930	ILLNFCLSILASNILILVGQSRV	952	PRIMARY	23
	5	963	AFLHFFFLSSFCWVLTEAWQSYL	985	SECONDARY	23
	6	1000	FLCLGWGLPALVVAVSVG	1017	SECONDARY	18
	7	1039	LYAFVGPAAVIVLVNMLIGIIVF	1061	PRIMARY	23
	8	1085	PWASLLLPCSACGAVPSPLLSSA	1107	SECONDARY	23
	9	1116	LWSSCVVLPLLALTWMSAVLAMT	1138	PRIMARY	23
	10	1143	VLFQALFAVFNSAQGFVITAVHC	1165	SECONDARY	23