ORK02612

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK02612

Order Kazusa clone(s) from :

Accession No
Product ID	ORK02612
Clone name	bm06951
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector (Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGCTGAAGCTTTCACAAGTTTGTAC AAAAAAGCAGGCTCTTC-5' end of cDNA, GAGCTCTCACCACTTTGTAC AAGAAAGCTGGGCGGCCGCTTTTTTTTTTTTTTTTTT-3' end of cDNA).
Symbol	CERS4
cDNA sequence	DNA sequence (2428 bp) Predicted protein sequence (420 aa)
HaloTag ORF Clone	FHC02612
Flexi ORF Clone	FXC02612
Description	LAG1 homolog, ceramide synthase 4

Features of the cloned cDNA sequence

Description

Length: 2428 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Features of the protein sequence

Description

Length: 420 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAG73339	8e-172	100.0	LAG1 homolog, c...	synthetic construct
Q9HA82	2e-171	99.7	LAG1 longevity ...	Homo sapiens
NP_078828	1.1e-170	99.2	LAG1 homolog, c...	Homo sapiens
EAW68940	2.3e-170	98.9	LAG1 longevity ...	Homo sapiens
BAD96341	9.2e-170	98.7	LAG1 longevity ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005547	177	375	PF03798	Longevity-assurance protein (LAG1)
HMMSmart	IPR006634	157	358	SM00724	TRAM
ProfileScan	IPR001356	110	154	PS50071	Homeobox
ProfileScan	IPR006634	157	358	PS50922	TRAM

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	160	CEASWRFLFYLSSFVGGLSVLY	181	SECONDARY	22
	2	205	LYWWYLLELGFYLSLLIRLPFDV	227	SECONDARY	23
	3	232	FKEQVIHHFVAVILMTFSYSANL	254	SECONDARY	23
	4	287	CDALFLIFSFVFFYTRLVLFPTQ	309	PRIMARY	23
	5	334	LLMLLQLLHVFWSCLILRMLYSF	356	PRIMARY	23