ORK04225

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK04225

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Accession No	AB209687
Product ID	ORK04225
Clone name	pf08939
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	ATP8A1
cDNA sequence	DNA sequence (8074 bp) Predicted protein sequence (1177 aa)
Flexi ORF Clone	FXC04225
Description	Probable phospholipid-transporting ATPase IA (EC 3.6.3.1) (Chromaffin granule ATPase II) (ATPase class I type 8A member 1).

Features of the cloned cDNA sequence

Description

Length: 8074 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	4539 bp
Genome contig ID	gi89161207r_42005151
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- TGTTATTAAAAATGAAATAAACTTTACCATTACTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAATTGTTGAAGTTTTGATTGCTAGTTCTAGAAATTATATTGCTTTCTA

Features of the protein sequence

Description

Length: 1177 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92924	100.0	ATPase, aminoph...	Homo sapiens
AAI09319	99.9	ATP8A1 protein ...	Homo sapiens
AAI09318	99.9	ATP8A1 protein ...	Homo sapiens
P70704	98.0	Probable phosph...	Mus musculus
Q29449	98.2	Probable phosph...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	435	449	PR00119	ATPase
	IPR001757	690	700	PR00119	ATPase
	IPR001757	812	831	PR00119	ATPase
HMMPfam	IPR008250	160	203	PF00122	E1-E2 ATPase-associated region
HMMPfam	IPR005834	431	835	PF00702	Haloacid dehalogenase-like hydrolase
HMMTigr	IPR006539	77	1099	TIGR01652	Phospholipid-translocating P-type ATPase
	IPR001757	127	229	TIGR01494	ATPase
	IPR001757	372	456	TIGR01494	ATPase
	IPR001757	665	715	TIGR01494	ATPase
	IPR001757	785	895	TIGR01494	ATPase
ScanRegExp	IPR001757	437	443	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	104	AANSFFLFIALLQQIPDVSPTGR	126	SECONDARY	23
	2	128	TTLVPLLFILAVAAIKEIIEDI	149	PRIMARY	22
	3	323	NVQILILFCILIAMSLVCSVGSA	345	PRIMARY	23
	4	370	LNFLTFIILFNNLIPISLLVTLE	392	PRIMARY	23
	5	772	QYFLDLALSCKAVICCRVSPLQK	794	SECONDARY	23
	6	869	SKCILYCFYKNIVLYIIEIWFAF	891	SECONDARY	23
	7	900	LFERWCIGLYNVMFTAMPPLTLG	922	SECONDARY	23
	8	954	FWVHCLNGLFHSVILFWFPLKAL	976	PRIMARY	23
	9	989	DYLLLGNFVYTFVVITVCLKAGL	1011	SECONDARY	23
	10	1019	FSHIAIWGSIALWVVFFGIYSSL	1041	PRIMARY	23
	11	1058	LFSSGVFWMGLLFIPVASLLLDV	1080	PRIMARY	23