ORK05355

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05355

Order Kazusa clone(s) from :

Accession No	AB209094
Product ID	ORK05355
Clone name	hj06473
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRIA1
cDNA sequence	DNA sequence (5461 bp) Predicted protein sequence (833 aa)
Description	Glutamate receptor 1 precursor (GluR-1) (GluR-A) (GluR-K1) (Glutamate receptor ionotropic, AMPA 1) (AMPA-selective glutamate receptor 1).

Features of the cloned cDNA sequence

Description

Length: 5461 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	2455 bp
Genome contig ID	gi51511721f_152750414
PolyA signal sequence (ATTAAA,-20)	+--------+--------+----*----+---- ACAGTTCTGGCTTTCATTAAATTTTGCTCTTTGGT
Flanking genome sequence (423020 - 423069)	----+--------+--------+--------+--------+----* ACCTGGGCCTTTTATTTAACATCTATATTTGTTTTAACTCTCTTGGCAGA

Features of the protein sequence

Description

Length: 833 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92331	100.0	glutamate recep...	Homo sapiens
EAW61651	100.0	glutamate recep...	Homo sapiens
BAF82254	99.7	unnamed protein...	Homo sapiens
XP_001503669	99.8	glutamate recep...	Equus caballus
XP_546286	99.7	similar to Glut...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	535	601	PD245531	NULL
FPrintScan	IPR001508	379	407	PR00177	NMDA receptor
	IPR001508	541	568	PR00177	NMDA receptor
	IPR001508	733	757	PR00177	NMDA receptor
HMMPfam	IPR001828	2	300	PF01094	Extracellular ligand-binding receptor
HMMPfam	IPR001320	463	754	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	335	710	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	460	DPLAYEIWMCIVFAYIGVSVVLF	482	PRIMARY	23
	2	511	EFGIFNSLWFSLGAFMQQGCDIS	533	SECONDARY	23
	3	546	WWFFTLIIISSYTANLAAFLTVE	568	PRIMARY	23
	4	731	SNVAGVFYILIGGLGLAMLVALI	753	PRIMARY	23