ORK05356

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05356

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Accession No	AB209092
Product ID	ORK05356
Clone name	hj05693
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRIA4
cDNA sequence	DNA sequence (5391 bp) Predicted protein sequence (801 aa)
Description	Glutamate receptor 4 precursor (GluR-4) (GluR4) (GluR-D) (Glutamate receptor ionotropic, AMPA 4) (AMPA-selective glutamate receptor 4).

Features of the cloned cDNA sequence

Description

Length: 5391 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	2791 bp
Genome contig ID	gi51511727f_104886649
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- CATATTGTAGGTGCTTAATAAATATTTGTTGAATG
Flanking genome sequence (471378 - 471427)	----+--------+--------+--------+--------+----* AATGAGTGAATTGAATTGAATTAATTTAATTAAGTGAAAGCAGAAAAACA

Features of the protein sequence

Description

Length: 801 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92329	100.0	glutamate recep...	Homo sapiens
EAW67069	100.0	glutamate recep...	Homo sapiens
EAW67066	100.0	glutamate recep...	Homo sapiens
EAW67067	100.0	glutamate recep...	Homo sapiens
EAW67068	100.0	glutamate recep...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	646	712	PD245531	NULL
FPrintScan	IPR001508	490	518	PR00177	NMDA receptor
FPrintScan	IPR001508	652	679	PR00177	NMDA receptor
HMMPfam	IPR001828	69	411	PF01094	Extracellular ligand-binding receptor
HMMPfam	IPR001320	574	801	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	446	799	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	571	DPLAYEIWMCIVFAYIGVSVVLF	593	PRIMARY	23
	2	622	EFGIFNSLWFSLGAFMQQGCDIS	644	SECONDARY	23
	3	657	WWFFTLIIISSYTANLAAFLTVE	679	PRIMARY	23