ORK05358

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05358

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Accession No	AB208918
Product ID	ORK05358
Clone name	hk05436
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRIN1
cDNA sequence	DNA sequence (4184 bp) Predicted protein sequence (934 aa)
Description	Glutamate [NMDA] receptor subunit zeta-1 precursor (N-methyl-D- aspartate receptor subunit NR1).

Features of the cloned cDNA sequence

Description

Length: 4184 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	1377 bp
Genome contig ID	gi89161216f_139053683
PolyA signal sequence (None)	+--------+--------+----*----+---- GCTCCTGTGGCCCACTCTGCCCCTGTCTCCCTGTG
Flanking genome sequence (126014 - 126063)	----+--------+--------+--------+--------+----* GCGGCCGCTCTGCCCAGCCCGCCCATGCTGCTCTCTCTCACTCTCTGGAC

Features of the protein sequence

Description

Length: 934 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92155	100.0	NMDA receptor 1...	Homo sapiens
CAM17671	100.0	glutamate recep...	Homo sapiens
Q05586	97.2	Glutamate [NMDA...	Homo sapiens
AAB59360	100.0	NMDA receptor s...	Homo sapiens
CAH72876	100.0	glutamate recep...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	650	719	PD245531	NULL
FPrintScan	IPR001508	497	525	PR00177	NMDA receptor
	IPR001508	587	612	PR00177	NMDA receptor
	IPR001508	625	649	PR00177	NMDA receptor
	IPR001508	656	683	PR00177	NMDA receptor
	IPR001508	838	862	PR00177	NMDA receptor
HMMPfam	IPR001828	130	383	PF01094	Extracellular ligand-binding receptor
HMMPfam	IPR001320	584	859	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	458	820	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	25	PMSTMRLLTLALLFSCSVARAAC	47	SECONDARY	23
	2	585	STLWLLVGLSVHVVAVMLYLLDR	607	PRIMARY	23
	3	626	LTLSSAMWFSWGVLLNSGIGEGA	648	SECONDARY	23
	4	658	GMVWAGFAMIIVASYTANLAAFL	680	PRIMARY	23
	5	840	GVFMLVAGGIVAGIFLIFIEIA	861	PRIMARY	22