ORK05359

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05359

Order Kazusa clone(s) from :

Accession No	AB208850
Product ID	ORK05359
Clone name	fj07108
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	GRIN2B
cDNA sequence	DNA sequence (4116 bp) Predicted protein sequence (1136 aa)
Description	Glutamate [NMDA] receptor subunit epsilon-2 precursor (N-methyl D- aspartate receptor subtype 2B) (NR2B) (NMDAR2B) (N-methyl-D-aspartate receptor subunit 3) (NR3) (hNR3).

Features of the cloned cDNA sequence

Description

Length: 4116 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	703 bp
Genome contig ID	gi89161190r_13506345
PolyA signal sequence (AAGAAA,-9)	+--------+--------+----*----+---- AAAAAAAGAAAGAAAGAAAAAGAGAGAAGAAAGAG
Flanking genome sequence (313684 - 313635)	----+--------+--------+--------+--------+----* TGACTAATGAACATGAAAAATATTTAGCCTCCCTGTAATCCTATAGAAAT

Features of the protein sequence

Description

Length: 1136 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92087	100.0	Glutamate [NMDA...	Homo sapiens
Q13224	100.0	Glutamate [NMDA...	Homo sapiens
AAB49993	99.9	N-methyl-D-aspa...	Homo sapiens
AAD00659	99.9	N-methyl-D-aspa...	Homo sapiens
XP_001088140	99.8	N-methyl-D-aspa...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	286	344	PD245531	NULL
FPrintScan	IPR001508	126	154	PR00177	NMDA receptor
	IPR001508	211	236	PR00177	NMDA receptor
	IPR001508	251	275	PR00177	NMDA receptor
	IPR001508	282	309	PR00177	NMDA receptor
	IPR001508	470	494	PR00177	NMDA receptor
HMMPfam	IPR001320	207	491	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	83	451	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	210	VWVMMFVMLLIVSAVAVFVFEYF	232	PRIMARY	23
	2	250	PSFTIGKAIWLLWGLVFNNSVP	271	SECONDARY	22
	3	287	WAFFAVIFLASYTANLAAFMIQE	309	SECONDARY	23
	4	470	MAGVFYMLGAAMALSLITFICEH	492	PRIMARY	23