ORK05569

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK05569

Order Kazusa clone(s) from :

Accession No	AB208930
Product ID	ORK05569
Clone name	aj00153
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	KCNC3
cDNA sequence	DNA sequence (4381 bp) Predicted protein sequence (428 aa)
Description	Potassium voltage-gated channel subfamily C member 3 (Voltage-gated potassium channel subunit Kv3.3) (KSHIIID).

Features of the cloned cDNA sequence

Description

Length: 4381 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : NO

Integrity of 3' end

Length of 3'UTR	3093 bp
Genome contig ID	gi42406306r_55407011
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- CACTCCCCACCAAAATAAACGTTTCCTTTTCATTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAATGGCCTCCTGTGTGCCTGTTCGGAAAGTCTCCTGATTGCGGCTGG

Features of the protein sequence

Description

Length: 428 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92167	1.5e-131	100.0	Shaw-related vo...	Homo sapiens
AAV80432	1.4e-130	99.0	voltage-gated p...	Rattus norvegicus
EAW71844	1.9e-130	100.0	potassium volta...	Homo sapiens
Q14003	1.9e-130	100.0	Potassium volta...	Homo sapiens
AAV80433	5e-130	98.8	voltage-gated p...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR005404	22	35	PR01582	Kv3.3 voltage-gated K+ channel
	IPR003091	50	73	PR00169	Voltage-dependent potassium channel
	IPR003091	76	96	PR00169	Voltage-dependent potassium channel
	IPR003091	118	144	PR00169	Voltage-dependent potassium channel
	IPR003968	123	131	PR01491	Kv channel
	IPR003968	147	161	PR01491	Kv channel
	IPR003091	147	170	PR00169	Voltage-dependent potassium channel
	IPR003091	187	209	PR00169	Voltage-dependent potassium channel
	IPR003091	216	242	PR00169	Voltage-dependent potassium channel
	IPR003968	227	238	PR01491	Kv channel
	IPR005404	277	293	PR01582	Kv3.3 voltage-gated K+ channel
HMMPfam	IPR005821	50	238	PF00520	Ion transport

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	48	LTYVEGVCVVWFTFEFLMRITFC	70	PRIMARY	23
	2	78	KSSLNIIDCVAILPFYLEVGLSG	100	PRIMARY	23
	3	146	NEFLLLIIFLALGVLIFATMIYY	168	SECONDARY	23
	4	187	FKNIPIGFWWAVVTMTTLGYGD	208	SECONDARY	22
	5	223	CALAGVLTIAMPVPVIVNNFGMY	245	SECONDARY	23