ORK06432

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06432

Order Kazusa clone(s) from :

Accession No	AB209114
Product ID	ORK06432
Clone name	hh12768
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	POMT2
cDNA sequence	DNA sequence (5647 bp) Predicted protein sequence (684 aa)
Description	Protein O-mannosyl-transferase 2 (EC 2.4.1.109) (Dolichyl-phosphate- mannose--protein mannosyltransferase 2).

Features of the cloned cDNA sequence

Description

Length: 5647 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	3590 bp
Genome contig ID	gi51511730r_76711054
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- AGCCACTGATTTTATAATAAATAACACTTGTTTTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AATGCCTTGTTGTCCTTGTACTTAATTGAATCCGACTTGCCCTCTGCTCC

Features of the protein sequence

Description

Length: 684 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92351	100.0	putative protei...	Homo sapiens
Q9UKY4	99.6	Protein O-manno...	Homo sapiens
AAF14118	99.5	putative protei...	Homo sapiens
XP_510091	99.5	putative protei...	Pan troglodytes
XP_001101522	97.0	putative protei...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003342	69	313	PF02366	Glycosyl transferase
HMMPfam	IPR003608	360	534	PF02815	MIR
HMMSmart	IPR003608	341	397	SM00472	MIR
	IPR003608	410	466	SM00472	MIR
	IPR003608	471	528	SM00472	MIR
ProfileScan	IPR003608	341	397	PS50919	MIR
	IPR003608	410	466	PS50919	MIR
	IPR003608	471	528	PS50919	MIR
ScanRegExp	IPR000215	102	112	PS00284	Protease inhibitor I4

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	59	FEAVGWWALLALVTLLSFATRFH	81	PRIMARY	23
	2	151	MRGFCAFLGSWLVPFAYLTVLDL	173	SECONDARY	23
	3	189	FDTGCLTLSQYILLDPILMFFIM	211	PRIMARY	23
	4	248	GVKFVGLFIILQVGLNTIADLWY	270	PRIMARY	23
	5	292	CLIVLPLALYTATFAVHFMVLSK	314	PRIMARY	23