ORK06522

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK06522

Order Kazusa clone(s) from :

Accession No	AB209495
Product ID	ORK06522
Clone name	ff06424
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	PTCH1
cDNA sequence	DNA sequence (10631 bp) Predicted protein sequence (586 aa)
Description	Protein patched homolog 1 (PTC1) (PTC).

Features of the cloned cDNA sequence

Description

Length: 10631 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: YES

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	7409 bp
Genome contig ID	gi89161216r_97145515
PolyA signal sequence (AATAAA,-27)	+--------+--------+----*----+---- CTAACATGAATAAAAAATAATATAACTTGTTGGTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATATCTTTGTTTGTAGAATGCTTTTTTTCTTTAAAATACTTGGGAGAGAC

Features of the protein sequence

Description

Length: 586 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD92732	100.0	patched variant...	Homo sapiens
CAH73818	99.6	patched homolog...	Homo sapiens
NP_001077071	99.6	protein patched...	Homo sapiens
EAW92632	99.6	patched homolog...	Homo sapiens
NP_001077072	99.6	protein patched...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003392	169	212	PF02460	Patched
HMMPfam	IPR003392	472	577	PF02460	Patched

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	12	KAAVVVVFNFAMVLLIFPAILSM	34	PRIMARY	23
	2	186	KVVVIFLFLGLLGVSLYGTTR	206	PRIMARY	21
	3	460	LRHWLLLFISVVLACTFLVCAVF	482	PRIMARY	23
	4	507	MGLIGIKLSAVPVVILIASVGIG	529	PRIMARY	23
	5	558	MFAPVLDGAVSTLLGVLMLAGSE	580	SECONDARY	23