ORK07016

Kazusa Clone (Original Type)

Gene/Protein Characteristic Table for ORK07016

Order Kazusa clone(s) from :

Accession No	AB209282
Product ID	ORK07016
Clone name	fk04706
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
Symbol	STMN1
cDNA sequence	DNA sequence (2936 bp) Predicted protein sequence (134 aa)
Description	Stathmin (Phosphoprotein p19) (pp19) (Oncoprotein 18) (Op18) (Leukemia-associated phosphoprotein p18) (pp17) (Prosolin) (Metablastin) (Protein Pr22).

Features of the cloned cDNA sequence

Description

Length: 2936 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis) for : cloned DNA seq.

Warning for N-terminal truncation: NO

Warning for coding interruption : YES

Integrity of 3' end

Length of 3'UTR	2517 bp
Genome contig ID	gi89161185r_25999646
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- GACTTGAATGGTTAATAAAACTGCACAGTGCTGTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* GGTGGCAGTGACTTCTTTTGAGTTAGGTTAATAAATCAAGCCATAGAGCC

Features of the protein sequence

Description

Length: 134 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD92519	6.1e-50	100.0	stathmin 1 vari...	Homo sapiens
XP_001114361	1.1e-27	94.6	similar to Stat...	Macaca mulatta
CAM12864	4.5e-19	92.9	stathmin 1/onco...	Homo sapiens
EDL80723	5.7e-19	92.9	rCG31023, isofo...	Rattus norvegicus
CAM12865	5.7e-19	70.5	stathmin 1/onco...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000956	30	48	PR00345	Stathmin
FPrintScan	IPR000956	59	87	PR00345	Stathmin
HMMPfam	IPR000956	26	130	PF00836	Stathmin
ScanRegExp	IPR000956	62	71	PS00563	Stathmin

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	4	SLSQLIVQNTLPVACLLFTMASS	26	SECONDARY	23
SOSUI2	2	114	CSYTVIITYASDWKEIPVLIY	134	PRIMARY	21